| MWexperimental | 60 | kDa |
| MWexpected | 57 | kDa |
|
log I(s)
2.06×102
2.06×101
2.06×100
2.06×10-1
|
s, nm-1
|
Conformation of polypyrimidine tract binding protein in solution.
Petoukhov MV, Monie TP, Allain FH, Matthews S, Curry S, Svergun DI, Structure 14(6):1021-7 (2006) Europe PMCSASDAR4 – PTB full
Polypyrimidine tract-binding protein 1|
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Fits and models
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Synchrotron SAXS
data from solutions of
PTB full
in
25 mM Tris 100 mM NaCl 5.0 mM DTT, pH 7.2
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a 1D Gas detector detector
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 6.4 and 20.4 mg/ml were measured
.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN
Tags:
X33
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