Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing.

De Marco V, Gillespie PJ, Li A, Karantzelis N, Christodoulou E, Klompmaker R, van Gerwen S, Fish A, Petoukhov MV, Iliou MS, Lygerou Z, Medema RH, Blow JJ, Svergun DI, Taraviras S, Perrakis A, Proc Natl Acad Sci U S A 106(47):19807-12 (2009) Europe PMC

SASDAV3 – Geminin:Cdt1 2:1 heterotrimer

Geminin
DNA replication factor Cdt1
MWexperimental 37 kDa
MWexpected 108 kDa
VPorod 70 nm3
log I(s) 1.50×102 1.50×101 1.50×100 1.50×10-1
Geminin DNA replication factor Cdt1 small angle scattering data  s, nm-1
ln I(s)
Geminin DNA replication factor Cdt1 Guinier plot ln 1.50×102 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Geminin DNA replication factor Cdt1 Kratky plot 1.104 0 3 sRg
Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Geminin DNA replication factor Cdt1 CRYSOL model

Synchrotron SAXS data from solutions of Geminin:Cdt1 2:1 heterotrimer in 25 mM Tris75 200 mM NaCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. Concentration = UNKNOWN

Tags: X33
Geminin
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   23.6 kDa
 
UniProt   O75496
Sequence   FASTA
 
DNA replication factor Cdt1 (Cdt1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   60.4 kDa
 
UniProt   Q9H211
Sequence   FASTA