Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.

Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M, PLoS Pathog 12(6):e1005660 (2016) Europe PMC

SASDAV8 – DEAD box helicase DDX3

DEAD box RNA helicase DDX3 (51-418)
MWexperimental 47 kDa
MWexpected 42 kDa
VPorod 80 nm3
log I(s) 4.24×103 4.24×102 4.24×101 4.24×100
DEAD box RNA helicase DDX3 (51-418) small angle scattering data  s, nm-1
ln I(s)
DEAD box RNA helicase DDX3 (51-418) Guinier plot ln 4.25×103 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
DEAD box RNA helicase DDX3 (51-418) Kratky plot 1.104 0 √3 sRg
p(r)
DEAD box RNA helicase DDX3 (51-418) pair distance distribution function Rg: 2.9 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DEAD box RNA helicase DDX3 (51-418) SASREF model
log I(s)
 s, nm-1
DEAD box RNA helicase DDX3 (51-418) GASBOR model
Synchrotron SAXS data from solutions of DEAD box helicase DDX3 in 50 mM HEPES 150 mM NaCl, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DEAD box RNA helicase DDX3 (51-418) (DDX3)
Mol. type   Protein
Organism   Yersinia enterocolitica
Olig. state   Monomer
Mon. MW   41.5 kDa