| MWI(0) | 10 | kDa |
| MWexpected | 15 | kDa |
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log I(s)
9.99×100
9.99×10-1
9.99×10-2
9.99×10-3
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s, nm-1
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Human alphacoronavirus non-structural protein Nsp10
Sven Falke, Al Kikhney.SASDAY8 – Human alphacoronavirus non-structural protein Nsp10 in the presence of Zn
HCoV-229E Non-structural protein 10|
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Fits and models
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Synchrotron SAXS
data from solutions of
Human alphacoronavirus non-structural protein Nsp10 in the presence of Zn
in
25 mM HEPES 280 mM NaCl 2 mM DTT 500 µM ZnCl2, pH 7.6
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.5 and 3.1 mg/ml were measured
at 10°C.
Eight successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Storage temperature = UNKNOWN
Tags:
X33
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