Signaling ammonium across membranes through an ammonium sensor histidine kinase.

Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H, Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA, Nat Commun 9(1):164 (2018) Europe PMC

SASDCQ9 – Ks-Amt5-HK (+10uM ATP)

Ammonium transporter histidine kinase domain
MWI(0) 29 kDa
MWexpected 30 kDa
VPorod 53 nm3
log I(s) 1.79×103 1.79×102 1.79×101 1.79×100
Ammonium transporter histidine kinase domain small angle scattering data  s, nm-1
ln I(s)
Ammonium transporter histidine kinase domain Guinier plot ln 1.80×103 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
Ammonium transporter histidine kinase domain Kratky plot 1.104 0 √3 sRg
p(r)
Ammonium transporter histidine kinase domain pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.7 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Ks-Amt5-HK (+10uM ATP) in 20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, pH 8 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Ammonium transporter histidine kinase domain (Ks-Amt5-HK)
Mol. type   Protein
Organism   Candidatus Kuenenia stuttgartiensis
Olig. state   Monomer
Mon. MW   30.0 kDa
 
UniProt   Q1Q357
Sequence   FASTA