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171 hits found for Mertens

SASDLZ9 – Streptococcus pneumoniae lipid II isoglutaminyl synthase MurT:GatD complex (no Zn2+)

Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurTLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD experimental SAS data
Streptococcus pneumoniae lipid II isoglutaminyl synthase MurT:GatD complex (no Zn2+) Rg histogram
Sample: Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT monomer, 52 kDa Streptococcus pneumoniae (strain … protein
Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD monomer, 29 kDa Streptococcus pneumoniae (strain … protein
Buffer: 50 mM Hepes, 10 mM MgCl2, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23
Unravelling the reaction mechanism of glutamate amidation in Staphylococcus aureus peptidoglycan PhD thesis, NOVA University Lisbon - (2022)
...Mertens HD
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 126 nm3

SASDM22 – Staphylococcus aureus lipid II isoglutaminyl synthase MurT:GatD complex

Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurTLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD experimental SAS data
Staphylococcus aureus lipid II isoglutaminyl synthase MurT:GatD complex Rg histogram
Sample: Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT monomer, 49 kDa Staphylococcus aureus (strain … protein
Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD monomer, 28 kDa Staphylococcus aureus (strain … protein
Buffer: 100 mM Tris-HCl, 500 mM NaCl, 10 mM MgCl2, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Nov 29
Unravelling the reaction mechanism of glutamate amidation in Staphylococcus aureus peptidoglycan PhD thesis, NOVA University Lisbon - (2022)
...Mertens HD
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 121 nm3

SASDC32 – Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB)

Ethylene Receptor 1 experimental SAS data
Ethylene Receptor 1 Cytosolic Domain (in 250 mM NDSB) Rg histogram
Sample: Ethylene Receptor 1 dimer, 129 kDa Arabidopsis thaliana protein
Buffer: 20 mM Tris-NDSB 150 mM NaCl 1mM DTT 250 mM NDSB, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 19
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Mertens HD, Mueller-Dieckmann J
RgGuinier 4.7 nm
Dmax 15.8 nm
VolumePorod 316 nm3

SASDC42 – Ethylene Receptor 1 Cytosolic Domain

Ethylene Receptor 1 experimental SAS data
Ethylene Receptor 1 Cytosolic Domain Rg histogram
Sample: Ethylene Receptor 1 dimer, 129 kDa Arabidopsis thaliana protein
Buffer: 20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSB, pH: 8.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 29
Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1). J Biol Chem 290(5):2644-58 (2015)
...Mertens HD, Mueller-Dieckmann J
RgGuinier 5.5 nm
Dmax 19.0 nm
VolumePorod 274 nm3

SASDB52 – Death associated protein kinase (wild-type)

Death associated protein kinase wild-type experimental SAS data
NONE model
Sample: Death associated protein kinase wild-type , 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.7 nm
Dmax 8.1 nm
VolumePorod 101 nm3

SASDG52 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 30 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 66.0 nm

SASDB62 – Death associated protein kinase (D220K mutant)

Death associated protein kinase (D220K mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (D220K mutant) , 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 89 nm3

SASDG62 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 50 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 81.5 nm

SASDB72 – Death associated protein kinase (Basic Loop mutant)

Death associated protein kinase (Basic Loop mutant) experimental SAS data
NONE model
Sample: Death associated protein kinase (Basic Loop mutant) , 37 kDa Homo sapiens protein
Buffer: 50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
...Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 78 nm3

SASDG72 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 100 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 111.0 nm

SASDF82 – Urokinase plasminogen activator surface receptor, uPAR H47C-N259C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF).

Urokinase plasminogen activator surface receptorUrokinase-type plasminogen activator (Amino Terminal Fragment) experimental SAS data
DAMMIN model
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Urokinase-type plasminogen activator (Amino Terminal Fragment) monomer, 16 kDa Homo sapiens protein
Buffer: 20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4,, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jun 18
Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond? J Biol Chem (2019)
...Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M
RgGuinier 2.6 nm
Dmax 8.2 nm
VolumePorod 102 nm3

SASDG82 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 200 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 147.0 nm

SASDF92 – Urokinase plasminogen activator surface receptor, uPAR, T51C-V70C

Urokinase plasminogen activator surface receptor experimental SAS data
DAMMIN model
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 1
Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond? J Biol Chem (2019)
...Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 55 nm3

SASDG92 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 30 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 66.0 nm

SASDFA2 – Urokinase plasminogen activator surface receptor, uPAR T51C-V70C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF).

Urokinase plasminogen activator surface receptorUrokinase-type plasminogen activator (Amino Terminal Fragment) experimental SAS data
DAMMIN model
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Urokinase-type plasminogen activator (Amino Terminal Fragment) monomer, 16 kDa Homo sapiens protein
Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 1
Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond? J Biol Chem (2019)
...Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 77 nm3

SASDGA2 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 50 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 105.0 nm

SASDFB2 – Urokinase plasminogen activator surface receptor, uPAR, K50C-V70C

Urokinase plasminogen activator surface receptor experimental SAS data
DAMMIN model
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5
Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond? J Biol Chem (2019)
...Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M
RgGuinier 2.5 nm
Dmax 9.3 nm
VolumePorod 66 nm3

SASDGB2 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 100 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 112.0 nm

SASDFC2 – Urokinase plasminogen activator surface receptor, uPAR K50C-V70C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF).

Urokinase plasminogen activator surface receptorUrokinase-type plasminogen activator (Amino Terminal Fragment) experimental SAS data
DAMMIN model
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Urokinase-type plasminogen activator (Amino Terminal Fragment) monomer, 16 kDa Homo sapiens protein
Buffer: 20 mM PBS, 5 %(v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5
Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond? J Biol Chem (2019)
...Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 66 nm3

SASDGC2 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 200 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
...Mertens H, Svergun D
Dmax 180.0 nm

SASDS93 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSA3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain

Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSB3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain

Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSC3 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSD3 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain

Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSE3 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain

Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.0 nm

SASDSF3 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain

Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSG3 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSH3 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain

Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSJ3 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain

Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSK3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSL3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSM3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSN3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 1 M NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSP3 – Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain

Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(26-45) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSQ3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain

Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDSR3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain

Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSS3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain

Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDST3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain

Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 1 M NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSU3 – Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain

Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(45-64) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDSV3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain

Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSW3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain

Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDSX3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain

Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDSY3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain

Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 1 M NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDSZ3 – Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain

Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(67-86) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDS24 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDS34 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDS44 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDS54 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein
Buffer: 20 mM Tris, 1 M NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDH64 – Plasmodium falciparum myosin essential light chain, N-terminal domain

Myosin essential light chain experimental SAS data
PYMOL model
Sample: Myosin essential light chain monomer, 9 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Oct 25
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 1.4 nm
Dmax 4.3 nm
VolumePorod 12 nm3

SASDS64 – Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain

Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(66-81) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa house mouse protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDH74 – Plasmodium falciparum myosin essential light chain, full-length

Myosin essential light chain experimental SAS data
Myosin essential light chain Kratky plot
Sample: Myosin essential light chain monomer, 16 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jun 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 23 nm3

SASDS74 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain

Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDH84 – Toxoplasma gondii myosin essential light chain 2

Myosin essential light chain 2 experimental SAS data
Myosin essential light chain 2 Kratky plot
Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.1 nm
Dmax 6.7 nm
VolumePorod 27 nm3

SASDS84 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain

Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDH94 – Toxoplasma gondii myosin essential light chain 2 (TgELC2) bound to myosin A C-terminus

Myosin essential light chain 2Myosin A experimental SAS data
PYMOL model
Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 3 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 8
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 1.7 nm
Dmax 5.5 nm
VolumePorod 28 nm3

SASDS94 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain

Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDHA4 – Trimeric complex of myosin A with myosin light chain (MLC1) and essential light chain (TgELC1) from Toxoplasma gondii

Toxoplasma gondii essential light chain 1Myosin AToxoplasma gondii myosin light chain, full length experimental SAS data
PYMOL model
Sample: Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Toxoplasma gondii myosin light chain, full length monomer, 24 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 69 nm3

SASDSA4 – Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain

Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(87-105) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDHB4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 66-210) and essential light chain (TgELC1) from Toxoplasma gondii

Myosin light chain TgMLC1, residues 66-210Toxoplasma gondii essential light chain 1Myosin A experimental SAS data
PYMOL model
Sample: Myosin light chain TgMLC1, residues 66-210 monomer, 17 kDa Toxoplasma gondii protein
Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 50 nm3

SASDSB4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain

Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDHC4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 70-210) and essential light chain (TgELC1) from Toxoplasma gondii

Toxoplasma gondii essential light chain 1Myosin AToxoplasma gondii myosin light chain, residues 70-210 experimental SAS data
PYMOL model
Sample: Toxoplasma gondii essential light chain 1 monomer, 15 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Toxoplasma gondii myosin light chain, residues 70-210 monomer, 17 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 47 nm3

SASDSC4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain

Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDHD4 – Trimeric complex of myosin A with myosin light chain (MLC1, residues 66-210) and essential light chain (TgELC2) from Toxoplasma gondii

Myosin essential light chain 2Myosin light chain TgMLC1, residues 66-210Myosin A experimental SAS data
PYMOL model
Sample: Myosin essential light chain 2 monomer, 15 kDa Toxoplasma gondii protein
Myosin light chain TgMLC1, residues 66-210 monomer, 17 kDa Toxoplasma gondii protein
Myosin A monomer, 5 kDa Toxoplasma gondii protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 50 nm3

SASDSD4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain

Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDHE4 – Trimeric complex of myosin A with myosin tail interacting protein (MTIP, residues 60-204) and essential light chain (PfELC) from Plasmodium falciparum

Myosin essential light chainPlasmodium falciparum myosin AMyosin A tail domain interacting protein experimental SAS data
Myosin essential light chain Plasmodium falciparum myosin A Myosin A tail domain interacting protein Kratky plot
Sample: Myosin essential light chain monomer, 16 kDa Plasmodium falciparum protein
Plasmodium falciparum myosin A monomer, 5 kDa Plasmodium falciparum protein
Myosin A tail domain interacting protein monomer, 17 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 30
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
...Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 2.7 nm
Dmax 10.7 nm
VolumePorod 51 nm3

SASDSE4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain

Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 1 M NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDHF4 – Lipoprotein lipase-GPIHBP1-monoclonal antibody (5D2) complex

Lipoprotein lipaseGlycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1Monoclonal Antibody Fragment 5D2 experimental SAS data
CORAL model
Sample: Lipoprotein lipase monomer, 50 kDa Homo sapiens protein
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 monomer, 15 kDa Homo sapiens protein
Monoclonal Antibody Fragment 5D2 monomer, 47 kDa Mus musculus protein
Buffer: 10 mM Tris, 150 mM NaCl, 4 mM CaCL2, 10% (v/v) Glycerol, 0.05% 0.8mM CHAPS, ,0.05 % (v/v) NaN3, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 14
Unfolding of monomeric lipoprotein lipase by ANGPTL4: Insight into the regulation of plasma triglyceride metabolism Proceedings of the National Academy of Sciences :201920202 (2020)
...Mertens H, Birrane G, Meiyappan M, Olivecrona G, Jørgensen T, Young S, Ploug M
RgGuinier 4.9 nm
Dmax 17.5 nm
VolumePorod 187 nm3

SASDSF4 – Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain

Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(82-96) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSG4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain

Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDSH4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain

Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSJ4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain

Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDKK4 – A-type ATP synthase

n-Dodecyl-β-D-MaltopyranosideA-type ATP synthaseMonoclonal Antibody Fragment experimental SAS data
OTHER model
Sample: n-Dodecyl-β-D-Maltopyranoside , 77 kDa synthetic construct
A-type ATP synthase monomer, 655 kDa Thermus thermophilus protein
Monoclonal Antibody Fragment dimer, 33 kDa Homo sapiens protein
Buffer: 20 mM Tris/HCl, 100 mM sucrose, 100 mM NaCl, 2 mM MgCl2, 10% glycerol, 0.05% b-DDM, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2013 Mar 3
MPBuilder: A PyMOL Plugin for Building and Refinement of Solubilized Membrane Proteins Against Small Angle X-ray Scattering Data Journal of Molecular Biology :166888 (2021)
...Mertens H
RgGuinier 8.0 nm
Dmax 27.0 nm
VolumePorod 1549 nm3

SASDSK4 – Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain

Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(106-128) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDSL4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSM4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSN4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSP4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 1 M NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Sep 15
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDSQ4 – Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain

Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(110-125) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDSR4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain

Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDES4 – ACA8 in stealth nanodisc (SANS, 100% D2O)

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-type experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli
Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol 1:206 (2018)
...Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 202 nm3

SASDSS4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain

Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDAT4 – uPAR wild-type

Urokinase plasminogen activator surface receptor experimental SAS data
DAMMIF model
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 69 nm3

SASDET4 – ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O)

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 (deuterated 75%) experimental SAS data
ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O) Rg histogram
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli
Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Calmodulin-7 (deuterated 75%) monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol 1:206 (2018)
...Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H
RgGuinier 4.3 nm
Dmax 15.0 nm
VolumePorod 217 nm3

SASDST4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain

Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDAU4 – uPAR H47C/N259C

Urokinase plasminogen activator surface receptor experimental SAS data
DAMMIF model
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.2 nm
Dmax 7.0 nm
VolumePorod 57 nm3

SASDEU4 – ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc (SANS, 100% D2O)

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Calmodulin-7 Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli
Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Calmodulin-7 monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol 1:206 (2018)
...Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H
RgGuinier 4.8 nm
Dmax 18.0 nm
VolumePorod 297 nm3

SASDSU4 – Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain

Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(129-146) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.3 nm

SASDAV4 – uPAR wild-type AE105 complex

Urokinase plasminogen activator surface receptorsynthetic peptide AE105 experimental SAS data
Urokinase plasminogen activator surface receptor synthetic peptide AE105 Kratky plot
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
synthetic peptide AE105 monomer, 1 kDa protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 61 nm3

SASDEV4 – ACA8 protein (apo) in stealth nanodisc

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-type experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli
Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol 1:206 (2018)
...Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H
RgGuinier 5.3 nm
Dmax 20.0 nm
VolumePorod 626 nm3

SASDSV4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain

Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.0 nm

SASDAW4 – uPAR wild-type AE234 complex

Urokinase plasminogen activator surface receptorsynthetic peptide AE234 experimental SAS data
Urokinase plasminogen activator surface receptor synthetic peptide AE234 Kratky plot
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
synthetic peptide AE234 monomer, 1 kDa protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 57 nm3

SASDEW4 – ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 experimental SAS data
Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Calcium-transporting ATPase 8, plasma membrane-type Calmodulin-7 Kratky plot
Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli
Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Calmodulin-7 monomer, Arabidopsis thaliana protein
Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol 1:206 (2018)
...Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H
RgGuinier 5.9 nm
Dmax 22.0 nm
VolumePorod 805 nm3

SASDSW4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain

Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDAX4 – uPAR wild-type ATF complex

Urokinase plasminogen activator surface receptorUrokinase-type plasminogen activator experimental SAS data
Urokinase plasminogen activator surface receptor Urokinase-type plasminogen activator Kratky plot
Sample: Urokinase plasminogen activator surface receptor monomer, 37 kDa Homo sapiens protein
Urokinase-type plasminogen activator monomer, 49 kDa Homo sapiens protein
Buffer: 25 mM Sodium Phosphate 5 % Glycerol 50 mM NaSO4, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Jun 10
A flexible multidomain structure drives the function of the urokinase-type plasminogen activator receptor (uPAR). J Biol Chem 287(41):34304-15 (2012)
Mertens HD, Kjaergaard M, Mysling S, Gårdsvoll H, Jørgensen TJ, Svergun DI, Ploug M
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 102 nm3

SASDSX4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain

Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.1 nm

SASDAY4 – fH1015

Factor H CCP modules 10 to 15 experimental SAS data
DAMMIF model
Sample: Factor H CCP modules 10 to 15 monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM Potassium Phosphate, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Dec 2
The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. J Mol Biol 395(1):105-22 (2010)
...Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 68 nm3

SASDSY4 – Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain

Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain experimental SAS data
Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain Rg histogram
Sample: Segment S(130-143) of the Neurofilament low intrinsically disordered tail domain monomer, 2 kDa Mus musculus protein
Buffer: 20 mM Tris, 3 M GdnHCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 20
Intramolecular structural heterogeneity altered by long-range contacts in an intrinsically disordered protein. Proc Natl Acad Sci U S A 120(30):e2220180120 (2023)
...Mertens HDT, Ehm T, Yahalom N, Golombek A, Schwartz T, Svergun DI, Saleh OA, Dzubiella J, Beck R
RgGuinier 1.2 nm

SASDAZ4 – fH1114

Factor H CCP modules 11 to 14 experimental SAS data
DAMMIF model
Sample: Factor H CCP modules 11 to 14 monomer, 28 kDa Homo sapiens protein
Buffer: 50 mM Potassium Phosphate, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Dec 2
The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. J Mol Biol 395(1):105-22 (2010)
...Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 38 nm3

SASDA25 – fh1213

Factor H CCP modules 12 to 13 experimental SAS data
CRYSOL model
Sample: Factor H CCP modules 12 to 13 monomer, 14 kDa Homo sapiens protein
Buffer: 50 mM Potassium Phosphate, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Dec 2
The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module. J Mol Biol 395(1):105-22 (2010)
...Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN
RgGuinier 2.0 nm
Dmax 7.1 nm
VolumePorod 20 nm3