Synchrotron SAXS
data from solutions of
Ammonium transporter Ks-Amt5 (Batch)
in
20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH 8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 1.00 mg/ml was measured
at 20°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Batch mode Ks-Amt5 sample in 0.09% (w/v) CYMAL-5 detergent. Pooled fractions from offline SEC.
s, nm-1
