Synchrotron SAXS data from solutions of TET12(1.6)S-c6b in 20 mM Tris 150 mM NaCl 10% glycerol, pH 7.5 were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. Scattering curves were merged and analyzed using PRIMUS software.
Concentration min = UNKNOWN
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TET12(1.6)S-c6b
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Mol. type |
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Protein |
Organism |
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synthetic construct |
Olig. state |
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Monomer |
Mon. MW |
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54.5 kDa |
Sequence |
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FASTA |
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