Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.

Ljubetič A, Lapenta F, Gradišar H, Drobnak I, Aupič J, Strmšek Ž, Lainšček D, Hafner-Bratkovič I, Majerle A, Krivec N, Benčina M, Pisanski T, Veličković TĆ, Round A, Carazo JM, Melero R, Jerala R
Nat Biotechnol 35(11):1094-1101 (2017 Nov)

PMID: 29035374 doi: 10.1038/nbt.3994

Submitted to SASBDB: 2017 Sep 28 Published in SASBDB: 2017 Oct 9

SASDBS9 – TET12SN Sample: TET12(1.10)SN-f5 monomer, 53 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Oct 28 RgGuinier 3.5 nm Dmax 12.0 nm VolumePorod 107 nm3

SASDCT7 – PYR16(4.6)SN-f5 Sample: PYR16(4.6)SN-f5 monomer, 72 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 16 RgGuinier 3.8 nm Dmax 11.3 nm VolumePorod 136 nm3

SASDCU7 – TRIP18(7.5R)SN-f5 Sample: TRIP18(7.5R)SN-f5 monomer, 82 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10 RgGuinier 4.2 nm Dmax 14.5 nm VolumePorod 210 nm3

SASDCR7 – TET12(1.10)SN-c6 Sample: TET12(1.10)SN-c6 monomer, 54 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10 RgGuinier 3.6 nm Dmax 11.5 nm VolumePorod 145 nm3

SASDCS7 – TET12(1.10)SN-f9 Sample: TET12(1.10)SN-f9 monomer, 57 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10 RgGuinier 3.9 nm Dmax 12.9 nm VolumePorod 153 nm3

SASDCV7 – TET12(2.3)SN-f5b Sample: TET12(2.3)SN-f5b monomer, 57 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10 RgGuinier 3.5 nm Dmax 11.5 nm VolumePorod 105 nm3

SASDCW7 – TET12(1.10)S-f5 Sample: TET12(1.10)S-f5 monomer, 53 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7 RgGuinier 3.6 nm Dmax 13.5 nm VolumePorod 210 nm3

SASDCX7 – TET12(1.10)S-f5b Sample: TET12(1.10)S-f5b monomer, 53 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7 RgGuinier 3.4 nm Dmax 11.2 nm VolumePorod 127 nm3

SASDCY7 – TET12(1.10)S-c6 Sample: TET12(1.10)S-c6 monomer, 55 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 7 RgGuinier 3.8 nm Dmax 13.0 nm VolumePorod 107 nm3

SASDCZ7 – TET12(1.6)S-c6b Sample: TET12(1.6)S-c6b monomer, 54 kDa synthetic construct protein Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Apr 7 RgGuinier 3.5 nm Dmax 11.5 nm VolumePorod 114 nm3