Quaternary Structure of Flavorubredoxin as Revealed by Synchrotron Radiation Small-Angle X-Ray Scattering

Petoukhov M, Vicente J, Crowley P, Carrondo M, Teixeira M, Svergun D, Structure 16(9):1428-1436 (2008) DOI

SASDLX4 – Full-length flavorubredoxin

Anaerobic nitric oxide reductase flavorubredoxin
MWexperimental 230 kDa
MWexpected 217 kDa
VPorod 297 nm3
log I(s) 3.59×102 3.59×101 3.59×100 3.59×10-1
Anaerobic nitric oxide reductase flavorubredoxin small angle scattering data  s, nm-1
ln I(s)
Anaerobic nitric oxide reductase flavorubredoxin Guinier plot ln 3.60×102 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Anaerobic nitric oxide reductase flavorubredoxin Kratky plot 1.104 0 3 sRg
p(r)
Anaerobic nitric oxide reductase flavorubredoxin pair distance distribution function Rg: 4.5 nm 0 Dmax: 14.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Full-length flavorubredoxin Rg histogram Rg, nm
Anaerobic nitric oxide reductase flavorubredoxin OTHER [STATIC IMAGE] model

Synchrotron SAXS data from solutions of flavorubredoxin in 50 mM Tris-HCl, 18% v/v glycerol, pH 7.5 were collected on the EMBL X33 beam line at DORIS III (DESY, Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 5 mg/ml were measured at 10°C. Two successive 60 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

The atomic coordinate files of the individual ensemble model representatives from the EOM cohort are made available in the full entry zip archive (represented here as a static image).

Tags: X33
Anaerobic nitric oxide reductase flavorubredoxin (norV)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Tetramer
Mon. MW   54.2 kDa
 
UniProt   Q46877 (1-479)
Sequence   FASTA