| MWexperimental | 131 | kDa |
| MWexpected | 136 | kDa |
| VPorod | 270 | nm3 |
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log I(s)
2.61×102
2.61×101
2.61×100
2.61×10-1
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s, nm-1
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X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB
Niemann H, Petoukhov M, Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D, Journal of Molecular Biology 377(2):489-500 (2008) DOISASDMT4 – Complex of Met receptor with InlB
Hepatocyte growth factor receptorInternalin B
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Fits and models
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Synchrotron SAXS
data from solutions of
Complex of Met receptor with InlB
in
25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a 1D Gas detector detector
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2 and 40 mg/ml were measured
.
15 successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN
Tags:
X33
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