Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules

Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D, Biochemistry 52(39):6844-6855 (2013) DOI

SASDMZ4 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml

Estrogen-related receptor gamma
Inverse repeat IR3 DNA
MWexperimental 100 kDa
MWexpected 276 kDa
log I(s) 4.74×101 4.74×100 4.74×10-1 4.74×10-2
Estrogen-related receptor gamma Inverse repeat IR3 DNA small angle scattering data  s, nm-1
ln I(s)
Estrogen-related receptor gamma Inverse repeat IR3 DNA Guinier plot ln 4.74×101 Rg: 4.7 nm 0 (4.7 nm)-2 s2
(sRg)2I(s)/I(0)
Estrogen-related receptor gamma Inverse repeat IR3 DNA Kratky plot 1.104 0 3 sRg
p(r)
Estrogen-related receptor gamma Inverse repeat IR3 DNA pair distance distribution function Rg: 4.6 nm 0 Dmax: 14.6 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Estrogen-related receptor gamma Inverse repeat IR3 DNA SASREF MX model

Synchrotron SAXS data from solutions of Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml in 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.90 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Tags: X33
Estrogen-related receptor gamma (Err)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Hexamer
Mon. MW   38.3 kDa
 
UniProt   P62508 (122-458)
Sequence   FASTA
 
Inverse repeat IR3 DNA (IR3)
Mol. type   DNA
Olig. state   Trimer
Mon. MW   15.5 kDa
Sequence   FASTA