| MWexperimental | 54 | kDa |
| MWexpected | 68 | kDa |
| VPorod | 110 | nm3 |
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log I(s)
9.55×105
9.55×104
9.55×103
9.55×102
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s, nm-1
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Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering.
Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI, J Biol Chem 284(37):25334-42 (2009) Europe PMCSASDN33 – Ternary Human Pex5p(C-terminal)-Pex14p(N)-PTS1 Complex (1:1:1 stoichiometry)
Peroxisomal targeting signal 1 receptor (C -terminal)Peroxisomal membrane protein PEX14 (N-terminal)
PTS1-BP
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Fits and models
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Synchrotron SAXS
data from solutions of
Ternary Human Pex5p(C-terminal)-Pex14p(N)-PTS1 Complex (1:1:1 stoichiometry)
in
50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.39 mg/ml was measured
at 15°C.
Two successive
120 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Tags:
X33
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