Metal-Mediated Self-Assembly of a β-Sandwich Protein

Crowley P, Matias P, Khan A, Roessle M, Svergun D, Chemistry - A European Journal 15(46):12672-12680 (2009) DOI

SASDND2 – The monomeric state of b-sandwich cupredoxin Plastocyanin (Pc)

Plastocyanin
MWexperimental 10 kDa
MWexpected 11 kDa
VPorod 15 nm3
log I(s) 1.54×103 1.54×102 1.54×101 1.54×100
Plastocyanin small angle scattering data  s, nm-1
ln I(s)
Plastocyanin Guinier plot ln 1.54×103 Rg: 1.4 nm 0 (1.4 nm)-2 s2
(sRg)2I(s)/I(0)
Plastocyanin Kratky plot 1.104 0 √3 sRg
p(r)
Plastocyanin pair distance distribution function Rg: 1.4 nm 0 Dmax: 4.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Plastocyanin PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of The monomeric state of b-sandwich cupredoxin Plastocyanin (Pc) in pure water, pH 7 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 20 mg/ml were measured . Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

Tags: X33
Plastocyanin
Mol. type   Protein
Organism   Phormidium laminosum
Olig. state   Monomer
Mon. MW   11.4 kDa
 
UniProt   Q51883
Sequence   FASTA
 
PDB ID   1BAW