Structural and functional characterization of soluble endoglin receptor

Le B, Franke D, Svergun D, Han T, Hwang H, Kim K, Biochemical and Biophysical Research Communications 383(4):386-391 (2009) DOI

SASDNZ2 – Tetrameric state of soluble endoglin receptor

Endoglin
MWexperimental 242 kDa
MWexpected 243 kDa
VPorod 434 nm3
log I(s) 6.94×102 6.94×101 6.94×100 6.94×10-1
Endoglin small angle scattering data  s, nm-1
ln I(s)
Endoglin Guinier plot ln 6.95×102 Rg: 7.6 nm 0 (7.6 nm)-2 s2
(sRg)2I(s)/I(0)
Endoglin Kratky plot 1.104 0 √3 sRg
p(r)
Endoglin pair distance distribution function Rg: 7.7 nm 0 Dmax: 26 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Endoglin DAMMIF model
log I(s)
 s, nm-1
Endoglin CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of Tetrameric state of soluble endoglin receptor in 10 mM Tris–HCl, 50 mM NaCl, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.05 mg/ml was measured at 25°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Endoglin
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   60.6 kDa
 
UniProt   P17813 (27-256)
Sequence   FASTA