Spiegelmers® NOX-E36 and NOX-A12

Al Kikhney, Bara Schmidt.

SASDPK2 – Spiegelmer NOX-A12, 3.5 mg/ml

Spiegelmer NOX-A12
MWexperimental 13 kDa
MWexpected 15 kDa
VPorod 18 nm3
log I(s) 4.51×101 4.51×100 4.51×10-1 4.51×10-2
Spiegelmer NOX-A12 small angle scattering data  s, nm-1
ln I(s)
Spiegelmer NOX-A12 Guinier plot ln 4.52×101 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Spiegelmer NOX-A12 Kratky plot 1.104 0 √3 sRg
p(r)
Spiegelmer NOX-A12 pair distance distribution function Rg: 2.1 nm 0 Dmax: 7.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Spiegelmer NOX-A12 MC-FOLD | MC-SYM model
Synchrotron SAXS data from solutions of Spiegelmer NOX-A12, 3.5 mg/ml in 20 mM HEPES, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was measured at 5.8°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Tags: X33
Spiegelmer NOX-A12
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   14.6 kDa
Sequence   FASTA