Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly.

Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R
Nucleic Acids Res (2022 May 30)

PMID: 35640611 doi: 10.1093/nar/gkac451

Submitted to SASBDB: 2021 Mar 24 Published in SASBDB: 2022 Jun 4

SASDL32 – Transcription elongation factor SPT6 Sample: Transcription elongation factor SPT6 monomer, 145 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 22 RgGuinier 4.5 nm Dmax 12.0 nm VolumePorod 249 nm3

SASDL22 – Transcription elongation factor SPT6 - ΔtSH2 variant Sample: Transcription elongation factor SPT6 - ΔtSH2 variant monomer, 122 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 22 RgGuinier 4.1 nm Dmax 13.9 nm VolumePorod 245 nm3

SASDKZ9 – Transcription elongation factor SPT6 - ΔN Spt6 Sample: Transcription elongation factor SPT6 - ΔN Spt6 variant monomer, 132 kDa Saccharomyces cerevisiae (strain … protein Buffer: 25 mM Hepes; 150 NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 4.7 nm Dmax 14.4 nm VolumePorod 288 nm3