Fine-tuning of intrinsic N-Oct-3 POU domain allostery by regulatory DNA targets

Alazard R, Mourey L, Ebel C, Konarev P, Petoukhov M, Svergun D, Erard M
Nucleic Acids Research 35(13):4420-4432 (2007 Jun 18)

doi: 10.1093/nar/gkm453 		Submitted to SASBDB: 2021 Jun 1
Published in SASBDB:

SASDL27 – N-Oct-3 POU transcription factor domain in complex with rat CRH DNA

POU domain, class 3, transcription factor 2Rat CRH DNA experimental SAS data
CUSTOM IN-HOUSE model
Sample: POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein
Rat CRH DNA monomer, 15 kDa Rattus norvegicus DNA
Buffer: 50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Oct 9
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 41 nm3

SASDL37 – N-Oct-3 POU transcription factor domain in complex with human DR-alpha DNA

POU domain, class 3, transcription factor 2Human DR-alpha DNA experimental SAS data
CUSTOM IN-HOUSE model
Sample: POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein
Human DR-alpha DNA monomer, 15 kDa Homo sapiens DNA
Buffer: 50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2004 May 26
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 44 nm3