Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex.

Butt BG, Owen DJ, Jeffries CM, Ivanova L, Hill CH, Houghton JW, Ahmed MF, Antrobus R, Svergun DI, Welch JJ, Crump CM, Graham SC
Elife 9 (2020 May 11)
PMID: 32391791
doi: 10.7554/eLife.53789 		Submitted to SASBDB: 2019 Oct 17
Published in SASBDB:

SASDG37 – 1:2 heterotrimer of pUL7 and pUL51(8-142) from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
DAMMIN model
Sample: Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 dimer, 30 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM tris, 200 mM NaCl, 3% (v/v) glycerol, 0.25 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 116 nm3

SASDG47 – 2:4 heterohexamer of pUL7 and pUL51 from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
DAMMIN model
Sample: Tegument protein UL7 dimer, 68 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 tetramer, 102 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
RgGuinier 4.6 nm
Dmax 19.7 nm
VolumePorod 340 nm3

SASDG57 – 1:2 heterotrimer of pUL7 and pUL51 from herpes simplex virus 1

Tegument protein UL7Tegument protein UL51 experimental SAS data
GASBOR model
Sample: Tegument protein UL7 monomer, 34 kDa Human alphaherpesvirus 1 … protein
Tegument protein UL51 dimer, 51 kDa Human alphaherpesvirus 1 protein
Buffer: 20 mM HEPES, 200 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 16
RgGuinier 4.0 nm
Dmax 18.2 nm
VolumePorod 160 nm3