Structural and biochemical analysis of Escherichia coli ObgE, a central regulator of bacterial persistence.

Gkekas S, Singh RK, Shkumatov AV, Messens J, Fauvart M, Verstraeten N, Michiels J, Versées W
J Biol Chem 292(14):5871-5883 (2017 Apr 7)

PMID: 28223358 doi: 10.1074/jbc.M116.761809

Submitted to SASBDB: 2016 Sep 28 Published in SASBDB: 2017 Feb 23

SASDBS6 – Apo form of full length ObgE from E.coli (ObgE_FL) Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17 RgGuinier 3.7 nm Dmax 18.1 nm VolumePorod 102 nm3

SASDBT6 – GppNHp bound form of full length ObgE from E.coli (ObgE_FL with GppNHp) Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17 RgGuinier 3.7 nm Dmax 14.5 nm VolumePorod 90 nm3

SASDBU6 – Apo form of the C-terminal deletion mutant of ObgE from E.coli (ObgE_340) Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein Buffer: 20 mM Hepes , 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Mar 12 RgGuinier 3.1 nm Dmax 11.6 nm VolumePorod 70 nm3

SASDBV6 – GppNHp bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GppNHp) Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 µM GppNHp, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Mar 12 RgGuinier 3.1 nm Dmax 11.4 nm VolumePorod 72 nm3

SASDBB9 – GDP bound form of full length ObgE from E.coli (ObgE_FL with GDP) Sample: GTPase ObgE/CgtA monomer, 44 kDa Escherichia coli protein Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17 RgGuinier 3.5 nm Dmax 16.0 nm VolumePorod 94673 nm3

SASDBC9 – GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP) Sample: GTPase ObgE/CgtA monomer, 39 kDa Escherichia coli protein Buffer: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP, pH: 7.5 Experiment: SAXS data collected at Rigaku BioSAXS-2000, on 2015 Feb 1 RgGuinier 3.0 nm Dmax 11.0 nm VolumePorod 70673 nm3