Browse by MACROMOLECULE type: protein

SASDTS5 – Sorting nexin-9, N-terminus - SNX9-SH3 (apo form)

Sorting nexin-9 experimental SAS data
Sorting nexin-9 Kratky plot
Sample: Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 42 nm3

SASDTT5 – SemDΔAPH bound to Sorting nexin-9, N-terminus - SNX9-SH3

Uncharacterized proteinSorting nexin-9 experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 94 nm3

SASDTU5 – SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL)

Uncharacterized proteinActin nucleation-promoting factor WASL experimental SAS data
SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL) Rg histogram
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 4.2 nm
Dmax 15.9 nm
VolumePorod 102 nm3

SASDUQ3 – C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV)

Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV) Rg histogram
Sample: Protein W monomer, 6 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
Pesce G, Gondelaud F, Ptchelkine D, Bignon C, Fourquet P, Longhi S
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 8 nm3

SASDUR3 – C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV)

Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV) Rg histogram
Sample: Protein W monomer, 6 kDa Henipavirus nipahense protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
Pesce G, Gondelaud F, Ptchelkine D, Bignon C, Fourquet P, Longhi S
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 8 nm3

SASDQA7 – Homodimeric luciferase EsLuxA

Alkanal monooxygenase alpha chain experimental SAS data
ALPHAFOLD model
Sample: Alkanal monooxygenase alpha chain dimer, 84 kDa Enhygromyxa salina protein
Buffer: 150 mM NaCl, 25 mM Tris, pH: 8
Experiment: SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Aug 1
luxA Gene From Enhygromyxa salina Encodes a Functional Homodimeric Luciferase Proteins: Structure, Function, and Bioinformatics (2024)
Yudenko A, Bazhenov S, Aleksenko V, Goncharov I, Semenov O, Remeeva A, Nazarenko V, Kuznetsova E, Fomin V, Konopleva M, Al Ebrahim R, Sluchanko N, Ryzhykau Y, Semenov Y, Kuklin A, Manukhov I, Gushchin I
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 138 nm3

SASDMN3 – Wild type E3 ubiquitin-protein ligase LRSAM1

E3 ubiquitin-protein ligase LRSAM1 experimental SAS data
DAMFILT model
Sample: E3 ubiquitin-protein ligase LRSAM1 monomer, 84 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Dec 7
Leucine Rich Repeat And Sterile Alpha Motif Containing 1 (LRSAM1)
Si Hoon Park
RgGuinier 6.1 nm
Dmax 19.4 nm
VolumePorod 250 nm3

SASDMP3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (1-632): C-terminal PTAP/PSAP motif and RING domain deletion mutant, ΔPTAP/PSAP-RING

E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING experimental SAS data
DAMFILT model
Sample: E3 ubiquitin-protein ligase LRSAM1 - ΔPTAP/PSAP-RING monomer, 74 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 1 mM TCEP, 5% w/v glycerol, pH: 8
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2017 Oct 24
Leucine Rich Repeat And Sterile Alpha Motif Containing 1 (LRSAM1)
Si Hoon Park
RgGuinier 5.5 nm
Dmax 18.0 nm
VolumePorod 322 nm3

SASDMQ3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (1-307): N-terminal LRR containing domain

E3 ubiquitin-protein ligase LRSAM1 - LRR containing N-terminal domain experimental SAS data
DAMFILT model
Sample: E3 ubiquitin-protein ligase LRSAM1 - LRR containing N-terminal domain monomer, 35 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 1 mM TCEP, 5% w/v glycerol, pH: 8
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2017 Oct 23
Leucine Rich Repeat And Sterile Alpha Motif Containing 1 (LRSAM1)
Si Hoon Park
RgGuinier 3.4 nm
Dmax 8.2 nm
VolumePorod 105 nm3

SASDMR3 – Wild type E3 ubiquitin-protein ligase LRSAM1 (561-632): SAM domain

E3 ubiquitin-protein ligase LRSAM1 - SAM domain experimental SAS data
DAMFILT model
Sample: E3 ubiquitin-protein ligase LRSAM1 - SAM domain monomer, 8 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2019 Apr 4
Leucine Rich Repeat And Sterile Alpha Motif Containing 1 (LRSAM1)
Si Hoon Park
RgGuinier 1.4 nm
Dmax 4.3 nm
VolumePorod 11 nm3