Curated repository for small angle scattering data and models


SASBDB currently contains:


3698

experimental data sets

5104

models
519 experimental data sets on hold
541 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDSH7 – Cationic liposomes containing DOTMA:DOPE (2:1) with negatively charged messenger RNA (mRNA) (raito 0.65:1)

mRNA -- proprietary sequence(R)-N,N,N-trimethyl-2-3-dioleyloxy-1-propanaminium chloride1,2-dioleoyl-sn-glycero-3-phosphoethanolamine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: MRNA -- proprietary sequence, 400 kDa RNA
(R)-N,N,N-trimethyl-2-3-dioleyloxy-1-propanaminium chloride, 1 kDa lipid
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, 1 kDa lipid
Buffer: 10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jun 9
Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H

Palivizumab Nanobody AIP1VHH complex
Palivizumab IgGAnti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data
Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry 2023 Sep :105270
Journal of Biological Chemistry :105270 (2023 Sep)
Precursor microRNA 31
Precursor microRNA 31 experimental SAS data
Structure of pre-miR-31 reveals an active role in Dicer-TRBP complex processing. Proc Natl Acad Sci U S A 2023 Sep 26;120(39):e2300527120
Proc Natl Acad Sci U S A 120(39):e2300527120 (2023 Sep 26)
Sulfite reductase flavoprotein crosslinked octamer
Sulfite reductase [NADPH] flavoprotein alpha-component experimental SAS data
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 2023 Sep 12;215(4):108028
J Struct Biol 215(4):108028 (2023 Sep 12)
MBP-, SUMO-fused heterodimeric SEPT6-SEPT7 septin coiled coil (MBP-SEPT6c+SUMO-SEPT7c)
Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP)Septin-7 (C-terminal domain of SEPT7 fused to SUMO) experimental SAS data
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol 2023 Oct 1;
Acta Crystallogr D Struct Biol (2023 Oct 1)
SARS-CoV-2 Main Protease H163A Mutant - 3.0 mg/mL
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 2023 Sep 12;14(1):5625
Nat Commun 14(1):5625 (2023 Sep 12)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.