Curated repository for small angle scattering data and models


SASBDB currently contains:


1896

experimental data sets

2890

models
499 experimental data sets on hold
486 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDJL7 – Oxidised fimbrial BcfH protein

Hypothetical exported protein experimental SAS data
CORAL model
Sample: Hypothetical exported protein trimer, 83 kDa Salmonella typhimurium (strain … protein
Buffer: 25 mM TRIS, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Aug 14
Salmonella enterica BcfH is a trimeric thioredoxin-like bifunctional enzyme with both thiol oxidase and disulfide isomerase activities. Antioxid Redox Signal (2021)
Subedi P, Paxman JJ, Wang G, Hor L, Hong Y, Verderosa AD, Whitten AE, Panjikar S, Santos-Martin CF, Martin JL, Totsika M, Heras B
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 93 nm3

Complement C5
Complement C5 experimental SAS data
The allosteric modulation of Complement C5 by knob domain peptides. Elife 2021 Feb 11;10
Elife 10 (2021 Feb 11)
BIP18SN
BIP18SN experimental SAS data
Self-assembly and regulation of protein cages from pre-organised coiled-coil modules. Nat Commun 2021 Feb 11;12(1):939
Nat Commun 12(1):939 (2021 Feb 11)
TET12SN(22CC)
TET12(1.10)SN-f5(22CC) experimental SAS data
Designed folding pathway of modular coiled-coil-based proteins. Nat Commun 2021 Feb 11;12(1):940
Nat Commun 12(1):940 (2021 Feb 11)
Ferric Iron Reductase - FhuF∆1-17
Ferric iron reductase protein FhuF (∆1-17) experimental SAS data
Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli JBIC Journal of Biological Inorganic Chemistry 2021 Feb 09
JBIC Journal of Biological Inorganic Chemistry (2021 Feb 09)
Haloalkane dehalogenase variant DhaA115 - monomeric fraction
Haloalkane dehalogenase variant DhaA115 -monomeric fraction experimental SAS data
Decoding the intricate network of molecular interactions of a hyperstable engineered biocatalyst Chemical Science 2020 11(41):11162-11178
Chemical Science 11(41):11162-11178 (2020)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.