Curated repository for small angle scattering data and models


SASBDB currently contains:


836

experimental data sets

1266

models
182 experimental data sets on hold
220 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDDT9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) in complex with Ras-related protein Rab 8 (MICAL1-Rab8 complex)

[F-actin]-monooxygenase MICAL1 (monomer)Ras-related protein 8 experimental SAS data
CORAL model
Sample: [F-actin]-monooxygenase MICAL1 (monomer)monomer, 118 kDa Homo sapiens protein
Ras-related protein 8monomer, 20 kDa protein
Buffer: 20 mM Hepes/NaOH, pH 7.5, 50 mM NaCl, 2 mM MgCl2, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 20
Human MICAL1: activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8. Protein Sci (2018)
Esposito A, Ventura V, Petoukhov MV, Rai A, Svergun DI, Vanoni MA
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 234 nm3

SASDEM4 – HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora
Type III secretion protein HrpGType III secretion protein HrpVHypersensitivity response secretion protein HrpJ experimental SAS data
CORAL model
Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion.
SASDD79 – High load concentration of alpha-aminoadipic semialdehyde dehydrogenase ALDH7A1 with nicotinamide adenine dinucleotide (NAD) collected by SEC-SAXS
Alpha-aminoadipic semialdehyde dehydrogenase experimental SAS data
PDB model
NAD+ Promotes Assembly of the Active Tetramer of Aldehyde Dehydrogenase 7A1.
SASDDA5 – Neurexin 1a L5L6 with ss6 insert
Neurexin 1a L5L6 with ss6 insert experimental SAS data
Neurexin 1a L5L6 with ss6 insert Rg histogram
Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer
SASDDP9 – C-terminal half of pseudorabies virus tegument protein UL37
Tegument protein UL37 experimental SAS data
DAMMIN model
The dynamic nature of the conserved tegument protein UL37 of herpesviruses.
SASDCH9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) with GDP
Sensory box/response regulator experimental SAS data
CORAL model
The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis

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