Curated repository for small angle scattering data and models


SASBDB currently contains:


2444

experimental data sets

3544

models
411 experimental data sets on hold
500 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDJ69 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 150 mM NaCl studied with SAXS

Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
MEMPROT model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Dec 8
Ambiguities in and completeness of SAS data analysis of membrane proteins: the case of the sensory rhodopsin II–transducer complex Acta Crystallographica Section D Structural Biology 77(11) (2021)
Ryzhykau Y, Vlasov A, Orekhov P, Rulev M, Rogachev A, Vlasova A, Kazantsev A, Verteletskiy D, Skoi V, Brennich M, Pernot P, Murugova T, Gordeliy V, Kuklin A
RgGuinier 7.6 nm
Dmax 41.0 nm

Complex of Archaeoglobus fulgidus Argonaute with dsDNA (5'p-ATTGTACGTACAAT)
Piwi protein AF_13185'-phosphorilated 14-mer DNA oligoduplex experimental SAS data
Prokaryotic Argonaute from Archaeoglobus fulgidus interacts with DNA as a homodimer. Sci Rep 2021 Feb 25;11(1):4518
Sci Rep 11(1):4518 (2021 Feb 25)
Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(48bp)
Upstream stimulatory factor 1Nuclear transcription factor Y subunit alphaNuclear transcription factor Y subunit betaNuclear transcription factor Y subunit gammaDNA 48bp experimental SAS data
The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding International Journal of Biological Macromolecules 2021 Oct
International Journal of Biological Macromolecules (2021 Oct)
Coronin from Trypanosoma brucei brucei (C-terminal coiled coil domain)
Coronin experimental SAS data
Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction Current Research in Structural Biology 2021 Oct
Current Research in Structural Biology (2021 Oct)
Two-component synthetic liposomes composed of 30% DOPS + 70% DOPC lipid mixtures loaded with M1 protein
Two-component synthetic liposomes composed of 30% DOPS + 70% DOPC lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) experimental SAS data
The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer Membranes 2021 Oct 10 11(10):772
Membranes 11(10):772 (2021 Oct 10)
Human Complex I assembly factor ACAD9 dimer
Complex I assembly factor ACAD9, mitochondrial experimental SAS data
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 2021 Oct 24(10):103153
iScience 24(10):103153 (2021 Oct)

Browse the contents according to:

Organism
Macromolecule type
Model type
Dissemination type
To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.