Curated repository for small angle scattering data and models


SASBDB currently contains:


1125

experimental data sets

1653

models
388 experimental data sets on hold
621 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDES6 – The 11S subunit of the Plasmodium falciparum proteasome, PA28

Proteasome activator PA28 experimental SAS data
CORAL model
Sample: Proteasome activator PA28 heptamer, 232 kDa Plasmodium falciparum (isolate ... protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 2
The structure of the PA28–20S proteasome complex from Plasmodium falciparum and implications for proteostasis Nature Microbiology (2019)
Xie S, Metcalfe R, Hanssen E, Yang T, Gillett D, Leis A, Morton C, Kuiper M, Parker M, Spillman N, Wong W, Tsu C, Dick L, Griffin M, Tilley L
RgGuinier 4.3 nm
Dmax 12.9 nm
VolumePorod 484 nm3

SASDFL3 – All 1H histone acetyltransferase Rtt109 complex with histones H3 and H4 and histone chaperones Asf1 and Vps75 (acquired in 100% v/v D2O)
Vacuolar protein sorting-associated protein 75 (1-225 aa)Histone acetyltransferase RTT109Histone chaperone ASF1Histone H3.2 (35-135 aa)Histone H4 experimental SAS data
HADDOCK model
Histone chaperone exploits intrinsic disorder to switch acetylation specificity.
SASDEE8 – Farnesylated human Guanylate Binding Protein 1 (farn-hGBP1), monomer from SEC-SAXS
farnesylated human Guanylate-binding protein 1 experimental SAS data
Farnesylated human Guanylate Binding Protein 1 (farn-hGBP1), monomer from SEC-SAXS Rg histogram
Farnesylation of human guanylate binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization.
SASDDA6 – Class I chitinase 2 from Agave tequilana
Chitinase 2 experimental SAS data
DAMMIF model
A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.
SASDEF9 – TraI of Neisseria gonorrhoeae
TraI experimental SAS data
GASBOR model
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island
SASDEL9 – Escherichia coli aerobic fatty acid beta-oxidation trifunctional enzyme complex
Fatty acid oxidation complex subunit alphaFatty acid oxidation complex subunit alpha3-ketoacyl-CoA thiolase FadA (beta subunit) experimental SAS data
DAMMIN model
Complementary substrate specificity and distinct quaternary assembly of the Escherichia coli aerobic and anaerobic beta-oxidation trifunctional enzyme complexes.

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