Curated repository for small angle scattering data and models


SASBDB currently contains:


624

experimental data sets

1048

models
182 experimental data sets on hold
160 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDBV3 – Dystrophin central domain repeats 16 to 21 (Δ2146-2305; Becker muscular dystrophy variant)

Dystrophin central domain repeats 16 to 21 (Δ2146-2305; Becker muscular dystrophy variant, deletion of exons 45-47) experimental SAS data
GASBOR model
Sample: Dystrophin central domain repeats 16 to 21 (Δ2146-2305; Becker muscular dystrophy variant, deletion of exons 45-47) monomer, Homo sapiens protein
Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol 5% acetonitrile, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Feb 5
Dystrophin's central domain forms a complex filament that becomes disorganized by in-frame deletions. J Biol Chem (2018)
Delalande O, Molza AE, Dos Santos-Morais R, Chéron A, Pollet É, Raguenes-Nicol C, Tascon C, Giudice E, Guilbaud M, Nicolas A, Bondon A, Leturcq F, Férey N, Baaden M, Perez J, Roblin P, Piétri-Rouxel F, Hubert JF, Czjzek M, Le Rumeur E
RgGuinier 6.0 nm
Dmax 21.0 nm
VolumePorod 184 nm3

SASDC95 – Light encoded DNA biosensor: e14A DNA
e14A experimental SAS data
DAMMIF model
Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.
SASDDS4 – cGMP-dependent protein kinase 1: ∆53 PKG Iα
cGMP-dependent protein kinase 1 experimental SAS data
DAMFILT model
An N-terminally truncated form of cyclic GMP-dependent protein kinase Iα (PKG Iα) is monomeric, autoinhibited, and provides a model for activation.
SASDCT6 – 12N12 nucleosome in 60% sucrose with ADP-BeF3
169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)Histone H2A type 1Histone H2B 1.1Histone H3.2Histone H4 experimental SAS data
12N12 nucleosome in 60% sucrose with ADP-BeF3 Rg histogram
The ATPase motor of the Chd1 chromatin remodeler stimulates DNA unwrapping from the nucleosome
SASDDY4 – Protein sex-lethal mutant with 10GS-linker
Protein sex-lethal mutant experimental SAS data
Protein sex-lethal mutant with 10GS-linker Rg histogram
A general small-angle X-ray scattering-based screening protocol validated for protein-RNA interactions.
SASDCV8 – Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum
Full-length hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.

Browse the contents according to:

Organism
Macromolecule type
Model type
Dissemination type