Curated repository for small angle scattering data and models


SASBDB currently contains:


2444

experimental data sets

3544

models
405 experimental data sets on hold
499 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDKV9 – Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(48bp)

Upstream stimulatory factor 1Nuclear transcription factor Y subunit alphaNuclear transcription factor Y subunit betaNuclear transcription factor Y subunit gammaDNA 48bp experimental SAS data
CORAL model
Sample: Upstream stimulatory factor 1 dimer, 50 kDa Homo sapiens protein
Nuclear transcription factor Y subunit alpha monomer, 10 kDa Homo sapiens protein
Nuclear transcription factor Y subunit beta monomer, 11 kDa Homo sapiens protein
Nuclear transcription factor Y subunit gamma monomer, 11 kDa Homo sapiens protein
DNA 48bp monomer, 30 kDa DNA
Buffer: 100 mM cacodylate buffer, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 14
The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding International Journal of Biological Macromolecules (2021)
Bernardini A, Lorenzo M, Chaves-Sanjuan A, Swuec P, Pigni M, Saad D, Konarev P, Graewert M, Valentini E, Svergun D, Nardini M, Mantovani R, Gnesutta N
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 155 nm3

Coronin from Trypanosoma brucei brucei (C-terminal coiled coil domain)
Coronin experimental SAS data
Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction Current Research in Structural Biology 2021 Oct
Current Research in Structural Biology (2021 Oct)
Two-component synthetic liposomes composed of 30% DOPS + 70% DOPC lipid mixtures loaded with M1 protein
Two-component synthetic liposomes composed of 30% DOPS + 70% DOPC lipid mixtures loaded with M1 protein (lipid:M1 molar ration 20:1) experimental SAS data
The Cytoplasmic Tail of Influenza A Virus Hemagglutinin and Membrane Lipid Composition Change the Mode of M1 Protein Association with the Lipid Bilayer Membranes 2021 Oct 10 11(10):772
Membranes 11(10):772 (2021 Oct 10)
Human Complex I assembly factor ACAD9 dimer
Complex I assembly factor ACAD9, mitochondrial experimental SAS data
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly iScience 2021 Oct 24(10):103153
iScience 24(10):103153 (2021 Oct)
Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tri-ubiquitin
Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell 2021 Sep 10;
Mol Cell (2021 Sep 10)
SWAXS data from Lysozyme Solution [Protein concentration 0.9 mg/ml] at 10 °C
Lysozyme C experimental SAS data
Visualizing how inclusion of higher reciprocal space in SWAXS data analysis improves shape restoration of biomolecules: case of lysozyme. J Biomol Struct Dyn 2021 Sep 25;:1-15
J Biomol Struct Dyn :1-15 (2021 Sep 25)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.