Curated repository for small angle scattering data and models


SASBDB currently contains:


1961

experimental data sets

2953

models
528 experimental data sets on hold
580 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDKM8 – Rationally optimised WA20 (ROWA) dimer

Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer experimental SAS data
DAMMIN model
Sample: Rationally optimised WA20 mutant N22A/H86K (ROWA) dimer dimer, 25 kDa Artificial protein (de … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2019 Nov 30
Rational thermostabilisation of four-helix bundle dimeric de novo proteins. Sci Rep 11(1):7526 (2021)
Irumagawa S, Kobayashi K, Saito Y, Miyata T, Umetsu M, Kameda T, Arai R
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 30 nm3

Transcriptional intermediary factor 2 (TIF2) intrinsically disordered coactivator
Nuclear receptor coactivator 2 experimental SAS data
Structural Insights into the Interaction of the Intrinsically Disordered Co-activator TIF2 with Retinoic Acid Receptor Heterodimer (RXR/RAR). J Mol Biol 2021 Feb 27;433(9):166899
J Mol Biol 433(9):166899 (2021 Feb 27)
Two titin I27 domains linked with GGG linker
Two titin I27 domains linked with GGG linker experimental SAS data
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 2021 Apr 23;550:43-48
Biochem Biophys Res Commun 550:43-48 (2021 Apr 23)
Human phenylalanine hydroxylase (hPAH)
Phenylalanine-4-hydroxylase experimental SAS data
Modulation of Human Phenylalanine Hydroxylase by 3-Hydroxyquinolin-2(1H)-One Derivatives Biomolecules 2021 Mar 19 11(3):462
Biomolecules 11(3):462 (2021 Mar 19)
DNA binding protein of starvation (DPS)
DNA protection during starvation protein experimental SAS data
Spatial Organization of Dps and DNA-Dps Complexes. J Mol Biol 2021 Mar 10;:166930
J Mol Biol :166930 (2021 Mar 10)
Beltless isolated translocation domain of tetanus nerotoxin (TeNT/blHn)
Tetanus toxin experimental SAS data
Structural flexibility of the tetanus neurotoxin revealed by crystallographic and solution scattering analyses. J Struct Biol X 2021;5:100045
J Struct Biol X 5:100045 (2021)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.