Curated repository for small angle scattering data and models


SASBDB currently contains:


844

experimental data sets

1275

models
232 experimental data sets on hold
301 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDD76 – Phox Homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α) in complex with inositol-hexaphosphate (IP6)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha experimental SAS data
DAMFILT model
Sample: Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha monomer, 33 kDa Homo sapiens protein
Buffer: 25 mM Tris 200 mM NaCl 5% Glycerol 0.5 mM TCEP 4 mM InsP6, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 20
Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α. Structure (2018)
Chen KE, Tillu VA, Chandra M, Collins BM
RgGuinier 2.6 nm
Dmax 9.3 nm
VolumePorod 48 nm3

SASDDM6 – Calbindin-D28K
Calbindin experimental SAS data
DAMMIF model
The X-ray structure of human calbindin-D28K: an improved model
SASDDG9 – The 2:1 complex of Synechocystis disulphide-trapped Fluorescence recovery protein dimer (CC mutant) and orange carotenoid-binding protein-ΔNTE (orange form)
Fluorescence recovery proteinOrange carotenoid-binding protein experimental SAS data
GASBOR model
OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.
SASDDT9 – NADPH oxidase (H2O2 producing and [F-actin] oxidizing) MICAL1 (monomer) in complex with Ras-related protein Rab 8 (MICAL1-Rab8 complex)
[F-actin]-monooxygenase MICAL1 (monomer)Ras-related protein 8 experimental SAS data
CORAL model
Human MICAL1: activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8.
SASDEM4 – HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora
Type III secretion protein HrpGType III secretion protein HrpVHypersensitivity response secretion protein HrpJ experimental SAS data
CORAL model
Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion.
SASDD79 – High load concentration of alpha-aminoadipic semialdehyde dehydrogenase ALDH7A1 with nicotinamide adenine dinucleotide (NAD) collected by SEC-SAXS
Alpha-aminoadipic semialdehyde dehydrogenase experimental SAS data
PDB model
NAD+ Promotes Assembly of the Active Tetramer of Aldehyde Dehydrogenase 7A1.

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