Curated repository for small angle scattering data and models


SASBDB currently contains:


3302

experimental data sets

4549

models
586 experimental data sets on hold
647 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDQ79 – Mutant 7-deazapurine in DNA protein A (D95A) bound to a 28 base pair DNA substrate (2-state model: complex plus free DNA)

Custom 28 base pair double stranded DNADNA-guanine transglycosylase - D95A mutant experimental SAS data
GROMACS model
Sample: Custom 28 base pair double stranded DNA monomer, 17 kDa Synthetic, purchased from … DNA
DNA-guanine transglycosylase - D95A mutant monomer, 50 kDa Salmonella enterica subsp. … protein
Buffer: 100 mM KCl, 50 mM Tris pH 7.0, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Dec 22
7-Deazaguanines in DNA: functional and structural elucidation of a DNA modification system. Nucleic Acids Res (2023)
Gedara SH, Wood E, Gustafson A, Liang C, Hung SH, Savage J, Phan P, Luthra A, de Crécy-Lagard V, Dedon P, Swairjo MA, Iwata-Reuyl D
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 104 nm3

Peptidyl-prolyl cis-trans isomerase AtFKBP43 (1-164)
Peptidyl-prolyl cis-trans isomerase FKBP43 experimental SAS data
The plant nucleoplasmin AtFKBP43 needs its extended arms for histone interaction. Biochim Biophys Acta Gene Regul Mech 2022 Oct;1865(7):194872
Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022 Oct)
Exon1 of Pathogenic form of Huntingtin (H46) with GFP fused at the C-terminus
Huntingtin experimental SAS data
The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity. Nat Struct Mol Biol 2023 Mar 2;
Nat Struct Mol Biol (2023 Mar 2)
Emfourin (M4in) from Serratia proteamaculans, M4 family peptidase inhibitor
Emfourin (Protealysin-associated protein) experimental SAS data
NMR structure of emfourin, a novel protein metalloprotease inhibitor: insights into the mechanism of action. J Biol Chem 2023 Mar 6;:104585
J Biol Chem :104585 (2023 Mar 6)
Desulfofustis sp. PB-SRB1 ligand-gated ion channel with calcium measured by paused-flow SEC-SANS
Neur_chan_LBD domain-containing protein experimental SAS data
Biophysical characterization of calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel. Proc Natl Acad Sci U S A 2022 Dec 13;119(50):e2210669119
Proc Natl Acad Sci U S A 119(50):e2210669119 (2022 Dec 13)
Growth factor receptor-bound protein 2 (GRB2) - N188D/N214D mutant monomer
Growth factor receptor-bound protein 2 (N188D, N214D) experimental SAS data
GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production. Sci Rep 2023 Mar 2;13(1):3505
Sci Rep 13(1):3505 (2023 Mar 2)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.