Curated repository for small angle scattering data and models


SASBDB currently contains:


3011

experimental data sets

4151

models
573 experimental data sets on hold
651 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDNH7 – Tn3 resolvase dimer bound to site II DNA sequence

Transposon Tn3 resolvaseTn3 res, resolvase binding site II experimental SAS data
CUSTOM IN-HOUSE model
Sample: Transposon Tn3 resolvase dimer, 43 kDa Escherichia coli protein
Tn3 res, resolvase binding site II monomer, 32 kDa DNA
Buffer: 25 mM Tris, 150 mM NaCl, 10 mM MgCl2, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 6
Structural basis for topological regulation of Tn3 resolvase. Nucleic Acids Res (2022)
Montaño SP, Rowland SJ, Fuller JR, Burke ME, MacDonald AI, Boocock MR, Stark WM, Rice PA
RgGuinier 5.0 nm
Dmax 14.9 nm
VolumePorod 217 nm3

Sperm-expressed surface protein
Sperm acrosome membrane-associated protein 6 experimental SAS data
SPACA6 ectodomain structure reveals a conserved superfamily of gamete fusion-associated proteins. Commun Biol 2022 Sep 17;5(1):984
Commun Biol 5(1):984 (2022 Sep 17)
Di[3-deoxy-D-manno-octulosonyl]-lipid A ammonium salt deposited onto silicon wafer (oriented sample anisotropic scattering, KDO2 control)
Di[3-deoxy-D-manno-octulosonyl]-lipid A (ammonium salt) experimental SAS data
Antimicrobial Peptide Mechanism Studied by Scattering-Guided Molecular Dynamics Simulation. J Phys Chem B 2022 Sep 6;
J Phys Chem B (2022 Sep 6)
single-stranded DNA dC->dU-editing enzyme APOBEC3G full length in complex with DNA: monomer
40-mer single stranded inhibitory DNADNA dC->dU-editing enzyme APOBEC-3G experimental SAS data
Small-Angle X-ray Scattering (SAXS) Measurements of APOBEC3G Provide Structural Basis for Binding of Single-Stranded DNA and Processivity Viruses 2022 Sep 06 14(9):1974
Viruses 14(9):1974 (2022 Sep 06)
Human histone deacetylase 6
Histone deacetylase 6 experimental SAS data
In-solution structure and oligomerization of human histone deacetylase 6 - an integrative approach. FEBS J 2022 Sep 5;
FEBS J (2022 Sep 5)
Meiosis protein TEX12 (Testis-expressed protein 12) – delta Ctip
Testis-expressed protein 12 experimental SAS data
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip Communications Biology 2022 Sep 07 5(1)
Communications Biology 5(1) (2022 Sep 07)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.