Curated repository for small angle scattering data and models


SASBDB currently contains:


3476

experimental data sets

4661

models
590 experimental data sets on hold
790 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDPD8 – Human apo iron-sulfur cluster assembly 1/iron-sulfur cluster assembly 2/NFU1 iron-sulfur cluster scaffold protein complex (ISCA1-ISCA2-NFU1)

Iron-sulfur cluster assembly 1 homolog, mitochondrialNFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G)Iron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
CORAL model
Sample: Iron-sulfur cluster assembly 1 homolog, mitochondrial monomer, 14 kDa Homo sapiens protein
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) monomer, 22 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial monomer, 13 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III, DESY on 2021 Mar 27
Structural plasticity of NFU1 upon interaction with binding partners: insights into the mitochondrial [4Fe-4S] cluster pathway Journal of Molecular Biology :168154 (2023)
Da Vela S, Saudino G, Lucarelli F, Banci L, Svergun D, Ciofi-Baffoni S
RgGuinier 3.2 nm
Dmax 11.7 nm
VolumePorod 70 nm3

Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme subunit alpha (anEcTFE-alpha)
Fatty acid oxidation complex subunit alpha experimental SAS data
Structural basis for different membrane-binding properties of E. coli anaerobic and human mitochondrial β-oxidation trifunctional enzymes. Structure 2023 May 11;
Structure (2023 May 11)
Monkeypox viral synthetic DNA fragment (DNA sequence 1, MP1)
Monekypox DNA sequence 1 experimental SAS data
Mapping and characterization of G‐quadruplexes in monkeypox genomes Journal of Medical Virology 2023 May 09 95(5)
Journal of Medical Virology 95(5) (2023 May 09)
Tn3 family transposase (TnpA WT)
TnpA transposase experimental SAS data
AFM-based force spectroscopy unravels stepwise formation of the DNA transposition complex in the widespread Tn3 family mobile genetic elements. Nucleic Acids Res 2023 Apr 7;
Nucleic Acids Res (2023 Apr 7)
invariant surface glycoprotein ISG65
65 kDa invariant surface glycoprotein, putative experimental SAS data
Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 2023 Apr 27;14(1):2403
Nat Commun 14(1):2403 (2023 Apr 27)
Astaxanthin-binding protein orange1 (AstaPo1) complexed with zeaxanthin
Astaxanthin binding fasciclin family protein experimental SAS data
Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP. Commun Biol 2023 Apr 28;6(1):471
Commun Biol 6(1):471 (2023 Apr 28)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.