Curated repository for small angle scattering data and models


SASBDB currently contains:


899

experimental data sets

1361

models
249 experimental data sets on hold
319 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDE57 – Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 4 mg/ml

Aldehyde dehydrogenase 16 from Loktanella sp. experimental SAS data
PYMOL model
Sample: Aldehyde dehydrogenase 16 from Loktanella sp. dimer, 161 kDa Loktanella sp. 3ANDIMAR09 protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, 2.0% glycerol, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Dec 13
Crystal Structure of Aldehyde Dehydrogenase 16 Reveals Trans-Hierarchical Structural Similarity and a New Dimer. J Mol Biol (2018)
Liu LK, Tanner JJ
RgGuinier 3.6 nm
Dmax 11.2 nm
VolumePorod 204 nm3

SASDDG2 – Glycosylated Human Immunoglobulin G Fc Region
Glycosylated human immunoglobulin G Fc region experimental SAS data
MODELLER model
CH2 domain orientation of human immunoglobulin G in solution: Structural comparison of glycosylated and aglycosylated Fc regions using small-angle X-ray scattering.
SASDDH3 – Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2)
Transcriptional regulator Lrs14-like protein experimental SAS data
DAMMIF model
Crystal structure of an Lrs14-like archaeal biofilm regulator from Sulfolobus acidocaldarius.
SASDET4 – ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O)
Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-typeCalmodulin-7 (deuterated 75%) experimental SAS data
ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O) Rg histogram
Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin
SASDED8 – Poly-L-Glutamic Acid in Dimethyl Sulfoxide (DMSO)
Poly-L-Glutamic Acid experimental SAS data
PYMOL model
β2-Type Amyloid-Like Fibrils of Poly-L-Glutamic Acid Convert into Long Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide
SASDEF2 – Mitochondrial import inner membrane translocase complex TIM9·10
Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CORAL model
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.

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