Curated repository for small angle scattering data and models


SASBDB currently contains:


755

experimental data sets

1136

models
164 experimental data sets on hold
194 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDCR9 – Kif2A-tubulin-DARPin complex in presence of AMP-PNP

Designed Ankyrin Repeat Protein D1Kinesin-like protein KIF2ATubulin alpha-1B chainTubulin beta-2B chain experimental SAS data
DAMMIF model
Sample: Designed Ankyrin Repeat Protein D1monomer, 18 kDa synthetic construct protein
Kinesin-like protein KIF2Amonomer, 48 kDa Homo sapiens protein
Tubulin alpha-1B chaindimer, 100 kDa Bos taurus protein
Tubulin beta-2B chaindimer, 100 kDa Bos taurus protein
Buffer: HEPES 20 mM, MgCl2 1mM, NaCl 150mM, pH: 7.2
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 May 16
Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate. Nat Commun 9(1):2628 (2018)
Trofimova D, Paydar M, Zara A, Talje L, Kwok BH, Allingham JS
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 374 nm3

SASDDB3 – Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)
Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier protein experimental SAS data
PDB model
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering.
SASDDD9 – Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain
cardiac myosin binding protein C: tri-helix bundle-C2 experimental SAS data
Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain Rg histogram
A Highly Conserved Yet Flexible Linker Is Part of a Polymorphic Protein-Binding Domain in Myosin-Binding Protein C.
SASDC75 – Leishmania braziliensis SGT co-chaperone
SGT protein experimental SAS data
DAMFILT model
Structural and functional studies of the Leishmania braziliensis SGT co-chaperone indicate that it shares structural features with HIP and can interact with both Hsp90 and Hsp70 with similar affinities.
SASDDB5 – MsbA in stealth nanodisc (SANS, 100% D2O)
Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
SASREF CV model
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs.
SASDDZ3 – p-hydroxyphenylacetate 3-hydroxylase, reductase component (mutant): 2 mg/ml of H170A C1 in the absence of p-hydroxyphenylacetic acid (HPA)
p-hydroxyphenylacetate 3-hydroxylase (HPAH), reductase component H170A mutant experimental SAS data
p-hydroxyphenylacetate 3-hydroxylase (HPAH), reductase component H170A mutant Kratky plot
Crystal structure of the flavin reductase of Acinetobacter baumannii p-hydroxyphenylacetate 3-hydroxylase (HPAH) and identification of amino acid residues underlying its regulation by aromatic ligands.

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