Curated repository for small angle scattering data and models


SASBDB currently contains:


692

experimental data sets

1113

models
192 experimental data sets on hold
162 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDDY2 – Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig

Leucine-rich repeat and fibronectin type-III domain-containing protein 4 experimental SAS data
Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig Rg histogram
Sample: Leucine-rich repeat and fibronectin type-III domain-containing protein 4dimer, 79 kDa Mus musculus protein
Buffer: 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3,, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 13
The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. Protein Eng Des Sel (2018)
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T
RgGuinier 3.7 nm
Dmax 12.1 nm
VolumePorod 183 nm3

SASDD93 – ATP-dependent Clp protease ATP-binding subunit ClpC1
ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
OTHER model
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis.
SASDDL3 – Folded ribonuclease A (RNAse)
Ribonuclease pancreatic experimental SAS data
PDB model
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.
SASDD65 – Ubiquitinating/deubiquitinating enzyme SdeA 207 914
Ubiquitinating/deubiquitinating enzyme SdeA experimental SAS data
OTHER model
Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination.
SASDB75 – Escherichia coli TraE protein: A VirB8 homolog from plasmid pKM101
Escherichia coli TraE protein (VirB8 homolog) experimental SAS data
GASBOR model
VirB8 homolog TraE from plasmid pKM101 forms a hexameric ring structure and interacts with the VirB6 homolog TraD.
SASDDH7 – Citrate-binding PAS domain from the sensor histidine kinase, CitA, fused to lipase EstA in the presence of citrate
citrate-binding CitAP domain fused to lipase A of Bacillus subtilis BsLA experimental SAS data
GROMACS model
A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase.

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