Curated repository for small angle scattering data and models


SASBDB currently contains:


1675

experimental data sets

2629

models
441 experimental data sets on hold
474 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDJR3 – BceAB-type ABC-Transporter nucleotide-binding domain, SaNsrF

ABC transporter, ATP-binding protein (Nucleotide-Binding Domain SaNsrF) experimental SAS data
GASBOR model
Sample: ABC transporter, ATP-binding protein (Nucleotide-Binding Domain SaNsrF) monomer, 31 kDa Streptococcus agalactiae protein
Buffer: 100 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2019 Dec 11
Characterization of the nucleotide-binding domain NsrF from the BceAB-type ABC-transporter NsrFP from the human pathogen Streptococcus agalactiae Scientific Reports 10(1) (2020)
Furtmann F, Porta N, Hoang D, Reiners J, Schumacher J, Gottstein J, Gohlke H, Smits S
RgGuinier 2.4 nm
Dmax 7.9 nm
VolumePorod 64 nm3

Type 3 secretion system pilotin, InvH27-147
Type 3 secretion system pilotin experimental SAS data
Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods. Structure 2020 Aug 27;
Structure (2020 Aug 27)
Adenylate cyclase toxin CyaA RTX block II-V
Bifunctional hemolysin/adenylate cyclase experimental SAS data
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol 2020 Aug 26;
J Mol Biol (2020 Aug 26)
Human Vitamin K-dependent Activated Protein C
Vitamin K-dependent protein C experimental SAS data
Zymogen and activated protein C have similar structural architecture. J Biol Chem 2020 Aug 27;
J Biol Chem (2020 Aug 27)
The retinoic acid receptor (RAR-RXR heterodimer) bound to the DNA response element HoxB13 DR0
Retinoic acid receptor alpha, RARRetinoic acid receptor RXR-alphaDNA response element HoxB13 DR0 experimental SAS data
Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR–RXR Nucleic Acids Research 2020 Aug 25
Nucleic Acids Research (2020 Aug 25)
Endonuclease VIII from E. coli (size exclusion chromatography SAXS)
Endonuclease 8 experimental SAS data
Unique Structural Features of Mammalian NEIL2 DNA Glycosylase Prime Its Activity for Diverse DNA Substrates and Environments. Structure 2020 Aug 15;
Structure (2020 Aug 15)

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Model type
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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated andvalidated repository for biological scattering data. Protein Science 29(1); 66-75.