Curated repository for small angle scattering data and models


SASBDB currently contains:


3843

experimental data sets

5294

models
534 experimental data sets on hold
422 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDSQ9 – NanoLuc luciferase

Oplophorus-luciferin 2-monooxygenase catalytic subunit experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Oplophorus-luciferin 2-monooxygenase catalytic subunit monomer, 20 kDa Oplophorus gracilirostris protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC - Masaryk University on 2021 May 27
Illuminating the mechanism and allosteric behavior of NanoLuc luciferase. Nat Commun 14(1):7864 (2023)
Nemergut M, Pluskal D, Horackova J, Sustrova T, Tulis J, Barta T, Baatallah R, Gagnot G, Novakova V, Majerova M, Sedlackova K, Marques SM, Toul M, Damborsky J, Prokop Z, Bednar D, Janin YL, Marek M
RgGuinier 1.8 nm
Dmax 5.9 nm
VolumePorod 38 nm3

Collagen-like peptide Mannan-binding lectin (MBL) center
Ac-(POG)4-QG-(POG)5-NH2 experimental SAS data
A solution structure analysis reveals a bent collagen triple helix in the complement activation recognition molecule mannan-binding lectin. J Biol Chem 2023 Feb;299(2):102799
J Biol Chem 299(2):102799 (2023 Feb)
24×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag
Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) experimental SAS data
Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 2024 Jan 690:149276
Biochemical and Biophysical Research Communications 690:149276 (2024 Jan)
I-actin - Skeletal muscle actin from Oryctolagus cuniculus: oligomer of time-inactivated actin (peak SEC fraction)
Actin, alpha skeletal muscle experimental SAS data
Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state Biochemical and Biophysical Research Communications 2023 Nov :149340
Biochemical and Biophysical Research Communications :149340 (2023 Nov)
Calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A)
Calmodulin-1Pre-mRNA-processing factor 40 homolog A experimental SAS data
Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A. Protein Sci 2023 Apr;32(4):e4606
Protein Sci 32(4):e4606 (2023 Apr)
Protease 1 from Pyrococcus horikoshii (PhP1) in Gd-HPDO3A
Deglycase PH1704 experimental SAS data
Medical contrast agents as promising tools for biomacromolecular SAXS experiments. Acta Crystallogr D Struct Biol 2022 Sep 1;78(Pt 9):1120-1130
Acta Crystallogr D Struct Biol 78(Pt 9):1120-1130 (2022 Sep 1)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.