Curated repository for small angle scattering data and models


SASBDB currently contains:


2776

experimental data sets

3917

models
510 experimental data sets on hold
652 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDME5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C) and Ras-related protein M-Ras

Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunitRas-related protein M-Ras experimental SAS data
BILBOMD model
Sample: Leucine-rich repeat protein SHOC-2 monomer, 65 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit monomer, 37 kDa Homo sapiens protein
Ras-related protein M-Ras monomer, 24 kDa Homo sapiens protein
Buffer: 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 20
Structural basis for SHOC2 modulation of RAS signalling. Nature (2022)
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M, Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J
RgGuinier 3.5 nm
Dmax 12.4 nm
VolumePorod 215 nm3

Accumulation-association protein (Aap) Brpt5.5 5xH85A dimer in the presence of ZnCl2
Accumulation associated protein (mutant) experimental SAS data
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol 2022 Jun 28;:167708
J Mol Biol :167708 (2022 Jun 28)
Botulinum Toxin E at acidic pH
Botulinum Toxin Serotype E, endonegative experimental SAS data
Elucidation of critical pH-dependent structural changes in Botulinum Neurotoxin E. J Struct Biol 2022 Jun 20;:107876
J Struct Biol :107876 (2022 Jun 20)
Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion
RNA recognition motif (RRM)-containing protein 4 experimental SAS data
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes PLOS Genetics 2022 Jun 21 18(6):e1010269
PLOS Genetics 18(6):e1010269 (2022 Jun 21)
Full length apo-polyketide synthase Pks13(S1533A) with Ser801 loaded by a C16-carbon chain
Polyketide synthase Pks13 experimental SAS data
Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 2022 Jun 21;20(1):147
BMC Biol 20(1):147 (2022 Jun 21)
A recoverin homology domain with EF hand domain of diacylglycerol kinase alpha under the condition without CaCl2
Diacylglycerol kinase alpha experimental SAS data
Ca 2+ ‐induced structural changes and intramolecular interactions in N‐terminal region of diacylglycerol kinase alpha Protein Science 2022 Jun 16 31(7)
Protein Science 31(7) (2022 Jun 16)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.