Curated repository for small angle scattering data and models


SASBDB currently contains:


2278

experimental data sets

3414

models
501 experimental data sets on hold
535 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDLF5 – RORg2 bound to a Variant-RORgamma Response Element

Retinoid-related orphan receptor-gammaVariant-RORgamma Response Element experimental SAS data
RORg2 bound to a Variant-RORgamma Response Element Rg histogram
Sample: Retinoid-related orphan receptor-gamma monomer, 56 kDa Homo sapiens protein
Variant-RORgamma Response Element dimer, 18 kDa Homo sapiens DNA
Buffer: 25 mM HEPES, 150 mM TCEP, 2% Glycerol, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 20
Conformational Changes of RORĪ³ During Response Element Recognition and Coregulator Engagement Journal of Molecular Biology :167258 (2021)
Strutzenberg T, Zhu Y, Novick S, Garcia-Ordonez R, Doebelin C, He Y, Ra Chang M, Kamenecka T, Edwards D, Griffin P
RgGuinier 4.4 nm
Dmax 22.1 nm
VolumePorod 112 nm3

Dengue Virus 2 New Guinea C (DENV-2 C, capsid protein) with saturating ST148 inhibitor
Dengue Virus 2 New Guinea C experimental SAS data
A cocrystal structure of dengue capsid protein in complex of inhibitor. Proc Natl Acad Sci U S A 2020 Jul 28;117(30):17992-18001
Proc Natl Acad Sci U S A 117(30):17992-18001 (2020 Jul 28)
Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with paused-flow SEC-SANS
Proton-gated ion channel experimental SAS data
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 2021 Sep 09 118(37):e2108006118
Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021 Sep 09)
200-310 region of Hendra virus P/V/W protein (PNT3)
Protein W experimental SAS data
Identification of a Region in the Common Amino-terminal Domain of Hendra Virus P, V, and W Proteins Responsible for Phase Transition and Amyloid Formation Biomolecules 2021 Sep 07 11(9):1324
Biomolecules 11(9):1324 (2021 Sep 07)
Ubiquitin activating enzyme 5 with ubiquitin-fold modifier 1 (UBA5 9 mg/mL + UFM1 2.25 mg/mL)
Ubiquitin-like modifier-activating enzyme 5Ubiquitin fold modifer 1 experimental SAS data
Structure and dynamics of UBA5-UFM1 complex formation showing new insights in the UBA5 activation mechanism Journal of Structural Biology 2021 Sep :107796
Journal of Structural Biology :107796 (2021 Sep)
LAF + P20 nanocomposite (NP added after fibrilization)
lysozyme amyloid fibrilFe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) experimental SAS data
Dependence of the Nanoscale Composite Morphology of Fe3O4 Nanoparticle-Infused Lysozyme Amyloid Fibrils on Timing of Infusion: A Combined SAXS and AFM Study Molecules 2021 Aug 11 26(16):4864
Molecules 26(16):4864 (2021 Aug 11)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.