Small Angle Scattering Biological Data Bank SASBDB

SASDFS7 – Talin-1 head amino acids 1-405(Δ139-168)

Talin-1 (Δ139-168), human experimental SAS data

Talin-1 head amino acids 1-405(Δ139-168) Rg histogram

Sample:	Talin-1 (Δ139-168), human monomer, 48 kDa Homo sapiens protein
Buffer:	50 mM sodium phosphate, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24

The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering. J Cell Sci 133(19) (2020)
Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Che...

R_g^Guinier	3.3	nm
D_max	13.3	nm
Volume^Porod	77	nm³

SASDFT7 – Talin-1 head amino acids 1-405

Talin-1 head amino acids 1-405 Rg histogram

Sample:	Talin-1, human monomer, 51 kDa Homo sapiens protein
Buffer:	50 mM sodium phosphate, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24

R_g^Guinier	3.4	nm
D_max	11.5	nm
Volume^Porod	94	nm³

SASDFU7 – Talin-1 head amino acids 1-405(Δ134-170/GAG insert)

Talin-1 (Δ134-170/GAG insert), human experimental SAS data

Talin-1 head amino acids 1-405(Δ134-170/GAG insert) Rg histogram

Sample:	Talin-1 (Δ134-170/GAG insert), human monomer, 47 kDa Homo sapiens protein
Buffer:	50 mM sodium phosphate, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24

R_g^Guinier	3.0	nm
D_max	10.2	nm
Volume^Porod	71	nm³

SASDJG5 – SARS-CoV-2 Main Protease

3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a experimental SAS data

Sample:	3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a dimer, 68 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 1 mM DTT, 1 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2020 Jun 1

COVID Main Protease
Nathanael Caveney

R_g^Guinier	2.7	nm
D_max	8.8	nm
Volume^Porod	93	nm³

SASDJH5 – SARS-CoV-2 Main Protease, P9T mutant

3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a, PT9 mutant experimental SAS data

Sample:	3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a, PT9 mutant monomer, 34 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 1 mM DTT, 1 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2020 Jun 1

COVID Main Protease
Nathanael Caveney

R_g^Guinier	2.4	nm
D_max	7.2	nm
Volume^Porod	54	nm³

SASDHR5 – Heat shock protein 40, subfamily A (PfHSP40)

HSP40, subfamily A experimental SAS data

Sample:	HSP40, subfamily A dimer, 97 kDa Plasmodium falciparum protein
Buffer:	50 mM Tris, 5 mM MgCl2, 300 mM KCl, 1 mM β-mercaptoethanol, 5% glycerol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24

Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding. Biochem J 477(18):3625-3643 (2020)
Anas M, Shukla A, Tripathi A, Kumari V, Prakash C, Nag P, Kumar LS, Sharma SK, Ramachandran R, Kumar N

R_g^Guinier	4.3	nm
D_max	15.0	nm
Volume^Porod	339	nm³

SASDHU5 – Heat shock protein 70 (PfHSP70-1)

Heat shock protein 70Peptide experimental SAS data

Sample:	Heat shock protein 70 monomer, 74 kDa Plasmodium falciparum protein Peptide monomer, 1 kDa synthetic construct protein
Buffer:	50mM Tris, 5mM MgCl2, 500mM KCl, 5mM Bme, 5% glycerol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24

R_g^Guinier	5.3	nm
D_max	16.6	nm
Volume^Porod	450	nm³

SASDHY5 – In cellulo luciferase protein crystals recombinantly expressed within High Five insect cells

Photinus pyralis firefly luciferase experimental SAS data

Photinus pyralis firefly luciferase Kratky plot

Sample:	Photinus pyralis firefly luciferase , 61 kDa Photinus pyralis protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase , 59 kDa Trypanosoma brucei brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH26 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data

Cathepsin B-like cysteine protease Kratky plot

Sample:	Cathepsin B-like cysteine protease , 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

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1684 hits found.