SASDR39 – MBP-, SUMO-fused heterodimeric SEPT6-SEPT7 septin coiled coil (MBP-SEPT6c+SUMO-SEPT7c)

Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP)Septin-7 (C-terminal domain of SEPT7 fused to SUMO) experimental SAS data
MULTIFOXS model
Sample: Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP) monomer, 55 kDa Homo sapiens protein
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.4 nm
Dmax 21.5 nm
VolumePorod 142 nm3

SASDR49 – MBP-, SUMO-fused heterodimeric SEPT8-SEPT7 septin coiled coil (MBP-SEPT8c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-8 (C-terminal domain of SEPT8 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-8 (C-terminal domain of SEPT8 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.3 nm
Dmax 19.9 nm
VolumePorod 140 nm3

SASDR59 – MBP-, SUMO-fused heterodimeric SEPT10-SEPT7 septin coiled coil (MBP-SEPT10c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-10 (C-terminal domain of SEPT10 fused to MBP) experimental SAS data
DAMMIN model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-10 (C-terminal domain of SEPT10 fused to MBP) monomer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.7 nm
Dmax 21.7 nm
VolumePorod 154 nm3

SASDR69 – MBP-, SUMO-fused heterodimeric SEPT11-SEPT7 septin coiled coil (MBP-SEPT11c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-11 (C-terminal domain of SEPT11 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-11 (C-terminal domain of SEPT11 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.2 nm
Dmax 20.9 nm
VolumePorod 139 nm3

SASDR79 – MBP-, SUMO-fused heterodimeric SEPT14-SEPT7 septin coiled coil (MBP-SEPT14c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-14 (C-terminal domain of SEPT14 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-14 (C-terminal domain of SEPT14 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.1 nm
Dmax 20.3 nm
VolumePorod 143 nm3

SASDS96 – Human Ubiquilin-1 (UBQLN1)

Ubiquilin-1 experimental SAS data
Ubiquilin-1 Kratky plot
Sample: Ubiquilin-1 dimer, 125 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22
UBQLN1, 2 & 4
Sarasi Galagedera
RgGuinier 4.6 nm
Dmax 19.3 nm
VolumePorod 264 nm3

SASDSA6 – Human Ubiquilin-2 (UBQLN2)

Ubiquilin-2 experimental SAS data
Ubiquilin-2 Kratky plot
Sample: Ubiquilin-2 dimer, 131 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
UBQLN1, 2 & 4
Sarasi Galagedera
RgGuinier 5.2 nm
Dmax 22.5 nm
VolumePorod 330 nm3

SASDSB6 – Human Ubiquilin-4 (UBQLN4)

Ubiquilin-4 experimental SAS data
Ubiquilin-4 Kratky plot
Sample: Ubiquilin-4 dimer, 128 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 26
UBQLN1, 2 & 4
Sarasi Galagedera
RgGuinier 5.1 nm
Dmax 21.7 nm
VolumePorod 333 nm3

SASDSG6 – Human Ubiquilin-2, ∆STI1-2 domain deletion (UBQLN2∆STI1-2)

Ubiquilin-2 (∆379-462) experimental SAS data
Ubiquilin-2 (∆379-462) Kratky plot
Sample: Ubiquilin-2 (∆379-462) monomer, 56 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
UBQLN2 domain deletion constructs_1
Sarasi Galagedera
RgGuinier 4.4 nm
Dmax 22.4 nm
VolumePorod 121 nm3

SASDSH6 – Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX)

Ubiquilin-2 (∆487-538) experimental SAS data
Ubiquilin-2 (∆487-538) Kratky plot
Sample: Ubiquilin-2 (∆487-538) dimer, 122 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
UBQLN2 domain deletion constructs_1
Sarasi Galagedera
RgGuinier 4.8 nm
Dmax 19.5 nm
VolumePorod 288 nm3

3704 hits found.