SASDV63 – B2 SINE ribozyme wildtype

B2 short interspaced nuclear element (SINE) RNA experimental SAS data
DAMMIN model
Sample: B2 short interspaced nuclear element (SINE) RNA monomer, 57 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 20
Diving into the structural architecture of the B2 SINE ribozyme
RgGuinier 5.4 nm
Dmax 17.3 nm
VolumePorod 131 nm3

SASDV73 – B2 SINE ribozyme with deletion mutation 81-124

B2 short interspaced nuclear element (SINE) RNA with deletion mutation 81-124 experimental SAS data
DAMMIF model
Sample: B2 short interspaced nuclear element (SINE) RNA with deletion mutation 81-124 dimer, 86 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Aug 19
Diving into the structural architecture of the B2 SINE ribozyme
Trushar Patel
RgGuinier 8.1 nm
Dmax 22.1 nm

SASDV83 – B2 SINE ribozyme with deletion mutation 96-105

B2 short interspaced nuclear element (SINE) RNA with deletion mutation 96-105 experimental SAS data
DAMMIN model
Sample: B2 short interspaced nuclear element (SINE) RNA with deletion mutation 96-105 monomer, 54 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Dec 18
Diving into the structural architecture of the B2 SINE ribozyme
Trushar Patel
RgGuinier 5.5 nm
Dmax 17.5 nm
VolumePorod 135 nm3

SASDV93 – B2 SINE ribozyme with B2J Mutation

B2 short interspaced nuclear element (SINE) RNA with two point mutations experimental SAS data
DAMMIN model
Sample: B2 short interspaced nuclear element (SINE) RNA with two point mutations monomer, 57 kDa Mus musculus RNA
Buffer: 5 mM Tris-HCl, 10 mM NaCl, 0.01% NP-40, 0.02 mM EDTA, 0.2 mM DTT, 0.5 mM MgCl2 and 1.5 % Glycerol, pH: 7.9
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Feb 20
Diving into the structural architecture of the B2 SINE ribozyme
Trushar Patel
RgGuinier 5.5 nm
Dmax 17.3 nm
VolumePorod 136 nm3

SASDR65 – ATP-dependent RNA helicase DDX3X (amino acids 1-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) experimental SAS data
ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) Kratky plot
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) monomer, 67 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 116 nm3

SASDR75 – ATP-dependent RNA helicase DDX3X (amino acids 50-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 50-580) experimental SAS data
ATP-dependent RNA helicase DDX3X (truncation; amino acids 50-580) Kratky plot
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 50-580) monomer, 62 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.6 nm
Dmax 12.9 nm
VolumePorod 102 nm3

SASDR85 – ATP-dependent RNA helicase DDX3X (amino acids 100-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 100-580) experimental SAS data
ATP-dependent RNA helicase DDX3X (truncation; amino acids 100-580) Kratky plot
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 100-580) monomer, 57 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.4 nm
Dmax 10.4 nm
VolumePorod 88 nm3

SASDR95 – ATP-dependent RNA helicase DDX3X (amino acids 135-580)

ATP-dependent RNA helicase DDX3X (truncation; amino acids 135-580) experimental SAS data
DAMFILT model
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 135-580) monomer, 52 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.1 nm
Dmax 9.2 nm
VolumePorod 77 nm3

SASDRA5 – 15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target)

15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) experimental SAS data
DAMMIF model
Sample: 15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) dimer, 10 kDa RNA
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 1.5 nm
Dmax 4.2 nm
VolumePorod 14 nm3

SASDRB5 – ATP-dependent RNA helicase DDX3X (amino acids 1-580) bound to a 15 nucleotide RNA duplex

ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580)15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) experimental SAS data
MONSA model
Sample: ATP-dependent RNA helicase DDX3X (truncation; amino acids 1-580) monomer, 67 kDa Homo sapiens protein
15 nucleotide RNA duplex (ATP-dependent RNA helicase DDX3X binding target) dimer, 10 kDa RNA
Buffer: 20 mM Tris, 150 mM NaCl, 10% (v/v) glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26
Solution structures of DEAD-box helicase DDX3X reveal the N-terminal extension binds RNA to modulate catalysis and influence conformation
Sarah Atkinson
RgGuinier 3.6 nm
Dmax 11.2 nm
VolumePorod 105 nm3

5116 hits found.