Small Angle Scattering Biological Data Bank SASBDB

SASDW94 – Horse apoferritin from AF4-SAXS measurements

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 480 kDa Equus caballus protein
Buffer:	phosphate buffered saline, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19

AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat (2025)
Da Vela S, Bartels K, Franke D, Soloviov D, Gräwert T, Molodenskiy D, Kolb B, Wilhelmy C, Drexel R, Meier F, Haas H, Langguth P, Graewert MA

R_g^Guinier	5.4	nm
D_max	13.3	nm
Volume^Porod	632	nm³

SASDWA4 – Yeast alcohol dehydrogenase 1 from AF4-SAXS measurements

Alcohol dehydrogenase 1 experimental SAS data

Sample:	Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	phosphate buffered saline, 1% glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13

R_g^Guinier	3.4	nm
D_max	9.5	nm
Volume^Porod	197	nm³

SASDWB4 – Sweet potato beta-amylase from AF4-SAXS measurement

Sample:	Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer:	phosphate buffered saline, 1% glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Apr 13

R_g^Guinier	4.2	nm
D_max	12.6	nm
Volume^Porod	297	nm³

SASDWC4 – 100 nm polystyrene beads from AF4-SAXS measurements

Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm experimental SAS data

Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm Kratky plot

Sample:	Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm monomer, 0 kDa synthetic construct
Buffer:	water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19

R_g^Guinier	47.7	nm
D_max	111.0	nm

SASDWD4 – 20 nm polystyrene beads from AF4-SAXS measurements

Thermo Fisher polystyrene nanospheres with a diameter of 20 nm experimental SAS data

Thermo Fisher polystyrene nanospheres with a diameter of 20 nm Kratky plot

Sample:	Thermo Fisher polystyrene nanospheres with a diameter of 20 nm monomer, 1 kDa synthetic construct
Buffer:	water/detergent solution (0.0125 % (v/v) NovaChem), pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Jun 19

R_g^Guinier	9.0	nm
D_max	25.0	nm

SASDX54 – Precursor microRNA 20a (pre-miR-20a)

Precursor microRNA 20a experimental SAS data

Sample:	Precursor microRNA 20a monomer, 23 kDa Homo sapiens RNA
Buffer:	50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, 5 mM BME, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 8

Structural features within precursor microRNA-20a regulate Dicer-TRBP processing Journal of Molecular Biology :169317 (2025)
Liu Y, Harkner C, Westwood M, Munsayac A, Keane S

R_g^Guinier	3.1	nm
D_max	11.2	nm
Volume^Porod	32	nm³

SASDV67 – Human Splicing Factor Proline/Glutamine rich SFPQ1 dimer with C-terminal truncation (delta599-707)

Splicing Factor Proline/Glutamine rich (1-598) experimental SAS data

Splicing Factor Proline/Glutamine rich (1-598) Kratky plot

Sample:	Splicing Factor Proline/Glutamine rich (1-598) dimer, 130 kDa Homo sapiens protein
Buffer:	500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2023 Mar 13

Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation Acta Crystallographica Section D Structural Biology 81(7):357-379 (2025)
Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C

R_g^Guinier	7.6	nm
D_max	34.3	nm
Volume^Porod	400	nm³

SASDV59 – Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

Splicing factor, proline- and glutamine-rich experimental SAS data

Splicing factor, proline- and glutamine-rich Kratky plot

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	150 mM KCl, 0.075% glycerol, 20 mM HEPES, 0.5 mM DTT,, pH: 7.4
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 20

R_g^Guinier	6.1	nm
D_max	22.0	nm
Volume^Porod	181	nm³

SASDXD4 – Full-length human SFPQ dimer (protiated and deuterated)

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	150 mM KCl, 1.5% glycerol, 20 mM HEPES, 1 mM DTT (in 100% H2O), pH: 7.4
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2023 Jan 23

R_g^Guinier	8.7	nm
D_max	30.7	nm
Volume^Porod	241000	nm³

SASDV57 – Full-length SFPQ in high salt buffer

Sample:	Splicing factor, proline- and glutamine-rich dimer, 153 kDa Homo sapiens protein
Buffer:	500mM KNO3, 20mM HEPES, 5% glycerol, 1mM DTT, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Oct 22

R_g^Guinier	7.9	nm
D_max	42.5	nm
Volume^Porod	485	nm³

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