SASDKL4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 10 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR
Yuki Toyama
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 250 nm3

SASDKM4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 5 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR
RgGuinier 3.8 nm
Dmax 15.0 nm
VolumePorod 213 nm3

SASDKN4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 2 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR
Yuki Toyama
RgGuinier 3.6 nm
Dmax 12.8 nm
VolumePorod 203 nm3

SASDKP4 – High temperature requirement of human mitochondrial serine protease HTRA2 (S306A mutant; 1 mg/ml)

Serine protease HTRA2, mitochondrial experimental SAS data
SASREF MX model
Sample: Serine protease HTRA2, mitochondrial hexamer, 210 kDa Homo sapiens protein
Buffer: 20 mM HEPES-NaOH (pH 7.4), 120 mM NaCl, 1 mM EDTA, and 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Jul 1
Oligomeric assembly regulating mitochondrial HtrA2 function as examined by methyl-TROSY NMR
Yuki Toyama
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 168 nm3

SASDKT2 – Wild type double Element for Nuclear Expression (dENE)

Wild type dENE experimental SAS data
Wild type dENE Kratky plot
Sample: Wild type dENE monomer, 23 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source (NSLS-II) on 2019 Jun 7
Structural Studies of Double Element for Nuclear Expression (dENE) with Poly(A) Tail
Yen-Lin Chen
RgGuinier 2.8 nm
Dmax 10.8 nm
VolumePorod 31 nm3

SASDKU2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)20

Wild type dENE + Poly(A)20 experimental SAS data
Wild type dENE + Poly(A)20 Kratky plot
Sample: Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source (NSLS-II) on 2019 Jun 7
Structural Studies of Double Element for Nuclear Expression (dENE) with Poly(A) Tail
Yen-Lin Chen
RgGuinier 2.8 nm
Dmax 10.7 nm

SASDKV2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)25

Wild type dENE + Poly(A)25 experimental SAS data
Wild type dENE + Poly(A)25 Kratky plot
Sample: Wild type dENE + Poly(A)25 monomer, 31 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source (NSLS-II) on 2019 Jun 7
Structural Studies of Double Element for Nuclear Expression (dENE) with Poly(A) Tail
Yen-Lin Chen
RgGuinier 2.9 nm
Dmax 10.4 nm

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30 experimental SAS data
Wild type dENE + Poly(A)30 Kratky plot
Sample: Wild type dENE + Poly(A)30 monomer, 33 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source (NSLS-II) on 2019 Jun 7
Structural Studies of Double Element for Nuclear Expression (dENE) with Poly(A) Tail
Yen-Lin Chen
RgGuinier 2.9 nm
Dmax 10.6 nm

SASDKX2 – double Element for Nuclear Expression (dENE) Mutant A

A dENE experimental SAS data
A dENE Kratky plot
Sample: A dENE monomer, 27 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source (NSLS-II) on 2019 Jun 7
Structural Studies of Double Element for Nuclear Expression (dENE) with Poly(A) Tail
Yen-Lin Chen
RgGuinier 3.2 nm
Dmax 10.9 nm

SASDKY2 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)20

A dENE + Poly(A)20 experimental SAS data
A dENE + Poly(A)20 Kratky plot
Sample: A dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source (NSLS-II) on 2019 Jun 7
Structural Studies of Double Element for Nuclear Expression (dENE) with Poly(A) Tail
Yen-Lin Chen
RgGuinier 3.2 nm
Dmax 11.5 nm

1918 hits found.