Small Angle Scattering Biological Data Bank SASBDB

SASDR39 – MBP-, SUMO-fused heterodimeric SEPT6-SEPT7 septin coiled coil (MBP-SEPT6c+SUMO-SEPT7c)

Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP)Septin-7 (C-terminal domain of SEPT7 fused to SUMO) experimental SAS data

Sample:	Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP) monomer, 55 kDa Homo sapiens protein Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC

R_g^Guinier	5.4	nm
D_max	21.5	nm
Volume^Porod	142	nm³

SASDR49 – MBP-, SUMO-fused heterodimeric SEPT8-SEPT7 septin coiled coil (MBP-SEPT8c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-8 (C-terminal domain of SEPT8 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-8 (C-terminal domain of SEPT8 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

R_g^Guinier	5.3	nm
D_max	19.9	nm
Volume^Porod	140	nm³

SASDR59 – MBP-, SUMO-fused heterodimeric SEPT10-SEPT7 septin coiled coil (MBP-SEPT10c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-10 (C-terminal domain of SEPT10 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-10 (C-terminal domain of SEPT10 fused to MBP) monomer, 56 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

R_g^Guinier	5.7	nm
D_max	21.7	nm
Volume^Porod	154	nm³

SASDR69 – MBP-, SUMO-fused heterodimeric SEPT11-SEPT7 septin coiled coil (MBP-SEPT11c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-11 (C-terminal domain of SEPT11 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-11 (C-terminal domain of SEPT11 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

R_g^Guinier	5.2	nm
D_max	20.9	nm
Volume^Porod	139	nm³

SASDR79 – MBP-, SUMO-fused heterodimeric SEPT14-SEPT7 septin coiled coil (MBP-SEPT14c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-14 (C-terminal domain of SEPT14 fused to MBP) experimental SAS data

Sample:	Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein Septin-14 (C-terminal domain of SEPT14 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 3

R_g^Guinier	5.1	nm
D_max	20.3	nm
Volume^Porod	143	nm³

SASDS96 – Human Ubiquilin-1 (UBQLN1)

Sample:	Ubiquilin-1 dimer, 125 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22

UBQLN1, 2 & 4
Sarasi Galagedera

R_g^Guinier	4.6	nm
D_max	19.3	nm
Volume^Porod	264	nm³

SASDSA6 – Human Ubiquilin-2 (UBQLN2)

Sample:	Ubiquilin-2 dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

UBQLN1, 2 & 4
Sarasi Galagedera

R_g^Guinier	5.2	nm
D_max	22.5	nm
Volume^Porod	330	nm³

SASDSB6 – Human Ubiquilin-4 (UBQLN4)

Sample:	Ubiquilin-4 dimer, 128 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 26

UBQLN1, 2 & 4
Sarasi Galagedera

R_g^Guinier	5.1	nm
D_max	21.7	nm
Volume^Porod	333	nm³

SASDSG6 – Human Ubiquilin-2, ∆STI1-2 domain deletion (UBQLN2∆STI1-2)

Ubiquilin-2 (∆379-462) experimental SAS data

Sample:	Ubiquilin-2 (∆379-462) monomer, 56 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

UBQLN2 domain deletion constructs_1
Sarasi Galagedera

R_g^Guinier	4.4	nm
D_max	22.4	nm
Volume^Porod	121	nm³

SASDSH6 – Human Ubiquilin-2, PXX domain deletion (UBQLN2∆PXX)

Ubiquilin-2 (∆487-538) experimental SAS data

Sample:	Ubiquilin-2 (∆487-538) dimer, 122 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

UBQLN2 domain deletion constructs_1
Sarasi Galagedera

R_g^Guinier	4.8	nm
D_max	19.5	nm
Volume^Porod	288	nm³

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3704 hits found.