|
|
|
Sample: |
Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP) monomer, 55 kDa Homo sapiens protein
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
|
Buffer: |
50 mM Tris, 150 mM NaCl,, pH: 7.5
|
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2023 Feb 3
|
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization.
Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
|
RgGuinier |
5.4 |
nm |
Dmax |
21.5 |
nm |
VolumePorod |
142 |
nm3 |
|
|
|
|
|
Sample: |
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-8 (C-terminal domain of SEPT8 fused to MBP) monomer, 55 kDa Homo sapiens protein
|
Buffer: |
50 mM Tris, 150 mM NaCl,, pH: 7.5
|
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2023 Feb 3
|
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization.
Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
|
RgGuinier |
5.3 |
nm |
Dmax |
19.9 |
nm |
VolumePorod |
140 |
nm3 |
|
|
|
|
|
Sample: |
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-10 (C-terminal domain of SEPT10 fused to MBP) monomer, 56 kDa Homo sapiens protein
|
Buffer: |
50 mM Tris, 150 mM NaCl,, pH: 7.5
|
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2023 Feb 3
|
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization.
Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
|
RgGuinier |
5.7 |
nm |
Dmax |
21.7 |
nm |
VolumePorod |
154 |
nm3 |
|
|
|
|
|
Sample: |
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-11 (C-terminal domain of SEPT11 fused to MBP) monomer, 55 kDa Homo sapiens protein
|
Buffer: |
50 mM Tris, 150 mM NaCl,, pH: 7.5
|
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2023 Feb 3
|
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization.
Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
|
RgGuinier |
5.2 |
nm |
Dmax |
20.9 |
nm |
VolumePorod |
139 |
nm3 |
|
|
|
|
|
Sample: |
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-14 (C-terminal domain of SEPT14 fused to MBP) monomer, 55 kDa Homo sapiens protein
|
Buffer: |
50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 7.5
|
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2023 Feb 3
|
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization.
Acta Crystallogr D Struct Biol (2023)
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
|
RgGuinier |
5.1 |
nm |
Dmax |
20.3 |
nm |
VolumePorod |
143 |
nm3 |
|
|
|
|
|
Sample: |
Ubiquilin-1 dimer, 125 kDa Homo sapiens protein
|
Buffer: |
20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
|
Experiment: |
SAXS
data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22
|
UBQLN1, 2 & 4
Sarasi Galagedera
|
RgGuinier |
4.6 |
nm |
Dmax |
19.3 |
nm |
VolumePorod |
264 |
nm3 |
|
|
|
|
|
Sample: |
Ubiquilin-2 dimer, 131 kDa Homo sapiens protein
|
Buffer: |
20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
|
Experiment: |
SAXS
data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
|
UBQLN1, 2 & 4
Sarasi Galagedera
|
RgGuinier |
5.2 |
nm |
Dmax |
22.5 |
nm |
VolumePorod |
330 |
nm3 |
|
|
|
|
|
Sample: |
Ubiquilin-4 dimer, 128 kDa Homo sapiens protein
|
Buffer: |
20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
|
Experiment: |
SAXS
data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 26
|
UBQLN1, 2 & 4
Sarasi Galagedera
|
RgGuinier |
5.1 |
nm |
Dmax |
21.7 |
nm |
VolumePorod |
333 |
nm3 |
|
|
|
|
|
Sample: |
Ubiquilin-2 (∆379-462) monomer, 56 kDa Homo sapiens protein
|
Buffer: |
20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
|
Experiment: |
SAXS
data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
|
UBQLN2 domain deletion constructs_1
Sarasi Galagedera
|
RgGuinier |
4.4 |
nm |
Dmax |
22.4 |
nm |
VolumePorod |
121 |
nm3 |
|
|
|
|
|
Sample: |
Ubiquilin-2 (∆487-538) dimer, 122 kDa Homo sapiens protein
|
Buffer: |
20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
|
Experiment: |
SAXS
data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
|
UBQLN2 domain deletion constructs_1
Sarasi Galagedera
|
RgGuinier |
4.8 |
nm |
Dmax |
19.5 |
nm |
VolumePorod |
288 |
nm3 |
|
|