SASDKD6 – NURS complex subunit red1

NURS complex subunit red1 experimental SAS data
NURS complex subunit red1 Kratky plot
Sample: NURS complex subunit red1 monomer, 8 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 20 mM HEPES, pH 7.5, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12
Red1 mediated sequestration of canonical polyA polymerase to MTREC leads to hyperadenylation of non-coding RNAs
Komal Soni
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 12 nm3

SASDKE6 – Poly(A) polymerase pla1 bound to the NURS complex subunit red1

NURS complex subunit red1Poly(A) polymerase pla1 experimental SAS data
CORAL model
Sample: NURS complex subunit red1 monomer, 8 kDa Schizosaccharomyces pombe (strain … protein
Poly(A) polymerase pla1 monomer, 65 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 20 mM HEPES, pH 7.5, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12
Red1 mediated sequestration of canonical polyA polymerase to MTREC leads to hyperadenylation of non-coding RNAs
Komal Soni
RgGuinier 3.3 nm
Dmax 10.7 nm
VolumePorod 138 nm3

SASDQM4 – CRISPR associated Lon protease (CalpL)

SAVED domain-containing protein experimental SAS data
DAMMIF model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14
Antiviral signaling by a cyclic nucleotide activated CRISPR protease Nature (2022)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter M, Boenigk W, Seifert R, Bode B, Schmid-Burgk J, Svergun D, Geyer M, White M, Hagelueken G
RgGuinier 3.1 nm
Dmax 10.3 nm
VolumePorod 80 nm3

SASDQN4 – Complex of CRISPR associated Lon protease (CalpL) with a 10 kDa C-terminal fragment of CalpT a putative MazF-like toxin (CalpT10)

SAVED domain-containing proteinUncharacterized protein (putative MazF-like toxin) experimental SAS data
CUSTOM IN-HOUSE model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Uncharacterized protein (putative MazF-like toxin) monomer, 9 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14
Antiviral signaling by a cyclic nucleotide activated CRISPR protease Nature (2022)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter M, Boenigk W, Seifert R, Bode B, Schmid-Burgk J, Svergun D, Geyer M, White M, Hagelueken G
RgGuinier 3.4 nm
Dmax 11.7 nm
VolumePorod 93 nm3

SASDQP4 – CRISPR associated Lon protease (CalpL) in the presence of cyclic oligoadenylate cA4

SAVED domain-containing protein experimental SAS data
DAMMIF model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Apr 8
Antiviral signaling by a cyclic nucleotide activated CRISPR protease Nature (2022)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter M, Boenigk W, Seifert R, Bode B, Schmid-Burgk J, Svergun D, Geyer M, White M, Hagelueken G
RgGuinier 3.2 nm
Dmax 10.3 nm
VolumePorod 82 nm3

SASDQQ4 – CRISPR associated Lon protease (CalpL) asymmetrically dimerizing in the presence of cyclic oligoadenylate cA4

SAVED domain-containing protein experimental SAS data
SASREF MX model
Sample: SAVED domain-containing protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Apr 8
Antiviral signaling by a cyclic nucleotide activated CRISPR protease Nature (2022)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter M, Boenigk W, Seifert R, Bode B, Schmid-Burgk J, Svergun D, Geyer M, White M, Hagelueken G
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 116 nm3

SASDP47 – Glucose Isomerase at a neutral pH and 5 MPa (1 atm) of pressure

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 253 nm3

SASDP57 – Glucose Isomerase at a neutral pH and 100 MPa (1000 atm) of pressure

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.5 nm
Dmax 10.6 nm
VolumePorod 267 nm3

SASDP67 – Bovine serum albumin at neutral pH, 4 °C, and 5 MPa (1 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.7 nm
Dmax 8.5 nm
VolumePorod 99 nm3

SASDP77 – Bovine serum albumin at neutral pH, 4 °C, and 100 MPa (1000 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 94 nm3

3112 hits found.