SASDTQ7 – RECQ like helicase 5 fragment (amino acids 625-825; 0.8 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5 , 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 4.6 nm
Dmax 13.2 nm
VolumePorod 102 nm3

SASDTR7 – RECQ like helicase 5 fragment (amino acids 625-825; 1.0 mg/ml)

ATP-dependent DNA helicase Q5 experimental SAS data
ATP-dependent DNA helicase Q5 Kratky plot
Sample: ATP-dependent DNA helicase Q5 , 109 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, CEITEC on 2023 Apr 19
Mechanisms of transcription attenuation and condensation of RNA polymerase II by RECQ5
Tomas Klumpler
RgGuinier 5.7 nm
Dmax 16.2 nm
VolumePorod 150 nm3

SASDVS6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245): 1666.7 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
SARS-CoV-2 N-protein variants: IDL176-246
Guillem Hernandez
RgGuinier 4.5 nm
Dmax 19.0 nm
VolumePorod 117 nm3

SASDVT6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data
Nucleoprotein (L223P, L227P, L230P) Kratky plot
Sample: Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 29
SARS-CoV-2 N-protein variants: IDL176-246
Guillem Hernandez
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 14 nm3

SASDVU6 – SARS-CoV-2 N-protein (N1-245; amino acids 1-245): 167 µM

Nucleoprotein experimental SAS data
Nucleoprotein Kratky plot
Sample: Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein
Buffer: 100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 13
SARS-CoV-2 N-protein variants: N1-246
Guillem Hernandez
RgGuinier 5.2 nm
Dmax 20.5 nm
VolumePorod 121 nm3

SASDVV9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in H₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVW9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 20 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 20% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVX9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 42 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 42% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVY9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 47 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetate, 47% v/v D₂O, pH: 5
Experiment: SANS data collected at D11, ILL on 2020 Aug 18
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVZ9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 66 % D₂O

β-chitin nanofibers from squid pens experimental SAS data
β-chitin nanofibers from squid pens Kratky plot
Sample: β-chitin nanofibers from squid pens None, Squid
Buffer: 20 mM acetic acid, 66% v/v D₂O, pH: 3.2
Experiment: SANS data collected at D11, ILL on 2019 Jul 5
Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

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