Α-Synuclein with K to R mutations and different NaCl concentration

Gil Koren.

SASD254 – α-Synuclein V1 2.52mg/ml 100 mM NaCl

Alpha-synuclein V1 K102R
MWexperimental 14 kDa
MWexpected 14 kDa
log I(s) 4.56×101 4.56×100 4.56×10-1 4.56×10-2
Alpha-synuclein V1 K102R small angle scattering data  s, nm-1
ln I(s)
Alpha-synuclein V1 K102R Guinier plot ln 4.56×101 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Alpha-synuclein V1 K102R Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of α-Synuclein V1 2.52mg/ml 100 mM NaCl in 20mM HEPES, pH 7.2 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099186 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.52 mg/ml was measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Alpha-synuclein V1 K102R
Mol. type   Protein
Olig. state   Monomer
Mon. MW   14.5 kDa
Sequence   FASTA