Synchrotron SAXS
data from solutions of
Recombinant CD2-specific cytoplasmic region of mouse protocadherin-15 in 10 mM NaCl
in
20 mM Tris, 10 mM NaCl, pH 8
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus3 2M detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.0992 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.3 and 8.2 mg/ml were measured
at 20°C.
10 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
s, nm-1
