Α-Synuclein with K to R mutations and different NaCl concentration

Gil Koren.

SASD2Q3 – α-Synuclein WT 1.71mg/ml 100 mM NaCl

Alpha-synuclein
MWexperimental 14 kDa
MWexpected 14 kDa
log I(s) 3.21×101 3.21×100 3.21×10-1 3.21×10-2
Alpha-synuclein small angle scattering data  s, nm-1
ln I(s)
Alpha-synuclein Guinier plot ln 3.22×101 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Alpha-synuclein Kratky plot 1.104 0 3 sRg

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of α-Synuclein WT 1.71mg/ml 100 mM NaCl in 20mM HEPES, pH 7.2 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099186 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.71 mg/ml was measured at 20°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Alpha-synuclein
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   14.5 kDa
 
UniProt   P37840
Sequence   FASTA