The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue.

Finci LI, Kr├╝ger N, Sun X, Zhang J, Chegkazi M, Wu Y, Schenk G, Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R, Neuron 83(4):839-849 (2014) Europe PMC

SASDA26 – DCC56

Deleted in Colorectal Cancer (FN5 & FN6)
MWexperimental 27 kDa
MWexpected 26 kDa
VPorod 38 nm3
log I(s) 1.28×103 1.28×102 1.28×101 1.28×100
Deleted in Colorectal Cancer (FN5 & FN6) small angle scattering data  s, nm-1
ln I(s)
Deleted in Colorectal Cancer (FN5 & FN6) Guinier plot ln 1.28×103 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Deleted in Colorectal Cancer (FN5 & FN6) Kratky plot 1.104 0 3 sRg
p(r)
Deleted in Colorectal Cancer (FN5 & FN6) pair distance distribution function Rg: 3.4 nm 0 Dmax: 11 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Deleted in Colorectal Cancer (FN5 & FN6) DAMMIN model

Synchrotron SAXS data from solutions of DCC56 in 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 9.9 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Deleted in Colorectal Cancer (FN5 & FN6) (dcc56)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   25.5 kDa
Sequence   FASTA