Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.

Tallant C, Valentini E Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A, Structure 23(1):80-92 (2015) Europe PMC

SASDA56 – BAZ2B in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminal
MWI(0) 33 kDa
MWexpected 28 kDa
VPorod 43 nm3
log I(s) 2.80×101 2.80×100 2.80×10-1 2.80×10-2
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal small angle scattering data  s, nm-1
ln I(s)
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal Guinier plot ln 2.80×101 Rg: 4 nm 0 (4 nm)-2 s2
(sRg)2I(s)/I(0)
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal Kratky plot 1.104 0 3 sRg
p(r)
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal pair distance distribution function Rg: 4.2 nm 0 Dmax: 14.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model

Synchrotron SAXS data from solutions of BAZ2B in Tris in 20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal (BAZ2B)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   27.7 kDa
Sequence   FASTA