data from solutions of
Varkud Satellite (VS) ribozyme in Tris
10 mM Tris 25 mM KCl 5 mM MgCl2, pH 7.5
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.5 and 4.5 mg/ml were measured
frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
SAXS data from a catalytically inactive construct support the overall conformation and dimeric state of the VS ribozyme in its x-ray crystal structure.