KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.

Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE, Nucleic Acids Res 43(20):10039-54 (2015) PubMed

SASDAN8 – mLBS1-2 DNA

MHV-68 TR DNA
MWexperimental 30 kDa
MWexpected 30 kDa
VPorod 50 nm3
log I(s) 1.78×103 1.78×102 1.78×101 1.78×100
MHV-68 TR DNA small angle scattering data  s, nm-1
ln I(s)
MHV-68 TR DNA Guinier plot ln 1.79×103 Rg: 4 nm 0 (4 nm)-2 s2
(sRg)2I(s)/I(0)
MHV-68 TR DNA Kratky plot 1.104 0 3 sRg
p(r)
MHV-68 TR DNA pair distance distribution function 0 Dmax: 16 nm

Data validation


Fits and models


log I(s)
 s, nm-1
MHV-68 TR DNA CRYSOL model

Synchrotron SAXS data from solutions of mLBS1-2 DNA in 10 mM TRIS 150 mM NaCl, pH 7.6 were collected on the EMBL P12 camera at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Study of oligomeric states of mLANA and kLANA and their complexes with DNA

MHV-68 TR DNA (mLBS1-2 )
Mol. type   DNA
Organism   unidentified herpesvirus
Olig. state   Monomer
Mon. MW   30 kDa