Conformation of polypyrimidine tract binding protein in solution.

Petoukhov MV, Monie TP, Allain FH, Matthews S, Curry S, Svergun DI, Structure 14(6):1021-7 (2006) Europe PMC

SASDAR4 – PTB full

Polypyrimidine tract-binding protein 1
MWexperimental 60 kDa
MWexpected 57 kDa
log I(s) 2.06×102 2.06×101 2.06×100 2.06×10-1
Polypyrimidine tract-binding protein 1 small angle scattering data  s, nm-1
ln I(s)
Polypyrimidine tract-binding protein 1 Guinier plot ln 2.06×102 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Polypyrimidine tract-binding protein 1 Kratky plot 1.104 0 3 sRg
p(r)
Polypyrimidine tract-binding protein 1 pair distance distribution function Rg: 4.4 nm 0 Dmax: 16.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Polypyrimidine tract-binding protein 1 BUNCH model

Synchrotron SAXS data from solutions of PTB full in 25 mM Tris 100 mM NaCl 5.0 mM DTT, pH 7.2 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a 1D Gas detector detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 6.4 and 20.4 mg/ml were measured . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Polypyrimidine tract-binding protein 1
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   57.2 kDa
 
UniProt   P26599
Sequence   FASTA