Synchrotron SAXS
data from solutions of
mLANA 124-316 mLBS1-2 8:1 complex
in
25 mM Na/K Phosphate, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.60 mg/ml was measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Study of oligomeric states of mLANA and kLANA and their complexes with DNA
|
MHV-68 TR DNA
(mLBS1-2 )
|
Mol. type |
|
DNA |
Organism |
|
unidentified herpesvirus |
Olig. state |
|
Monomer |
Mon. MW |
|
30 kDa |
Latency-associated nuclear antigen
(mLANA)
|
Mol. type |
|
Protein |
Organism |
|
Murid herpesvirus 4 |
Olig. state |
|
Octamer |
Mon. MW |
|
33.6 kDa |
|
UniProt |
|
O41974
(124-316)
|
Sequence |
|
FASTA |
|
|