KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.

Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE, Nucleic Acids Res 43(20):10039-54 (2015) PubMed

SASDAR8 – mLANA 124-316 mLBS1-2 8:1 complex

MHV-68 TR DNA
Latency-associated nuclear antigen
MWI(0) 290 kDa
MWexpected 299 kDa
VPorod 475 nm3
log I(s) 1.17×104 1.17×103 1.17×102 1.17×101
MHV-68 TR DNA Latency-associated nuclear antigen small angle scattering data  s, nm-1
ln I(s)
MHV-68 TR DNA Latency-associated nuclear antigen Guinier plot ln 1.18×104 Rg: 5.8 nm 0 (5.8 nm)-2 s2
(sRg)2I(s)/I(0)
MHV-68 TR DNA Latency-associated nuclear antigen Kratky plot 1.104 0 √3 sRg
p(r)
MHV-68 TR DNA Latency-associated nuclear antigen pair distance distribution function Rg: 6 nm 0 Dmax: 20 nm

Data validation

Fits and models

log I(s)
 s, nm-1
MHV-68 TR DNA Latency-associated nuclear antigen CRYSOL model
Synchrotron SAXS data from solutions of mLANA 124-316 mLBS1-2 8:1 complex in 25 mM Na/K Phosphate, pH 7.5 were collected on the EMBL P12 camera at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.60 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Study of oligomeric states of mLANA and kLANA and their complexes with DNA

MHV-68 TR DNA (mLBS1-2 )
Mol. type   DNA
Organism   unidentified herpesvirus
Olig. state   Monomer
Mon. MW   30 kDa
Latency-associated nuclear antigen (mLANA)
Mol. type   Protein
Organism   Murid herpesvirus 4
Olig. state   Octamer
Mon. MW   33.6 kDa
 
UniProt   O41974 (124-316)
Sequence   FASTA