Functional and structural insight into properdin control of complement alternative pathway amplification.

Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR, EMBO J 2017 Mar 6; PubMed

SASDB59 – Recombinant monomeric human Properdin

Human recombinant Properdin TSR 0-3
Human recombinant Properdin TSR 4-6
MWexperimental 50 kDa
MWexpected 49 kDa
log I(s) 1.68×104 1.68×103 1.68×102 1.68×101
Human recombinant Properdin TSR 0-3 Human recombinant Properdin TSR 4-6 small angle scattering data  s, nm-1
ln I(s)
Human recombinant Properdin TSR 0-3 Human recombinant Properdin TSR 4-6 Guinier plot ln 1.69×104 Rg: 5.1 nm 0 (5.1 nm)-2 s2
(sRg)2I(s)/I(0)
Human recombinant Properdin TSR 0-3 Human recombinant Properdin TSR 4-6 Kratky plot 1.104 0 3 sRg
Dmax: 18 nm
log I(s)
 s, nm-1
Human recombinant Properdin TSR 0-3 Human recombinant Properdin TSR 4-6 CORAL model
Synchrotron SAXS data from solutions of recombinantly formed monomeric human properdin in 10 mM HEPES pH 7.2, 150 mM NaCl, were collected EMBL-P12 bioSAXS beam line at the PETRAIII storage ring (Hamburg, Germany) equipped with a Pilatus 2M detector (I(s) vs s, where s = 4π sin θ/λ; 2θ is the scattering angle; λ = 0.124 nm). Solute concentrations ranging between 2.7 and 9.1 mg/ml were measured at 20°C. 20 successive 0.05 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Human recombinant Properdin TSR 0-3 (FP TSR 0-3)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   25.5 kDa
 
UniProt   P27918
Sequence   FASTA
 
Human recombinant Properdin TSR 4-6 (FP TSR 4-6)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   23.9 kDa
 
UniProt   P27918
Sequence   FASTA