Self-processing module of Iron-regulated protein FrpC

Vojtěch Kubáň.

SASDB85 – Self-processing module of iron-regulated protein FrpC (415-591)

Iron-regulated protein FrpC (amino acids 415-591)
MWexperimental 18 kDa
MWexpected 19 kDa
VPorod 21 nm3
log I(s) 1.40×10-1 1.40×10-2 1.40×10-3 1.40×10-4
Iron-regulated protein FrpC (amino acids 415-591) small angle scattering data  s, nm-1
ln I(s)
Iron-regulated protein FrpC (amino acids 415-591) Guinier plot ln 1.40×10-1 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
Iron-regulated protein FrpC (amino acids 415-591) Kratky plot 1.104 0 3 sRg
p(r)
Iron-regulated protein FrpC (amino acids 415-591) pair distance distribution function Rg: 1.6 nm 0 Dmax: 4.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Iron-regulated protein FrpC (amino acids 415-591) OTHER model

In-house small-angle X-ray scattering data from solutions of iron-regulated protein FrpC (amino acids 415-591) in 5 mM Tris 50 mM NaCl, 10 mM CaCl2, 0.1% NaN3, pH 7.4 were collected using a Rigaku BioSAXS-1000 SAXS instrument (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.154 nm). Different solute concentrations in the range 1.25-5.00 mg/ml were measured. Four successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were obtained from a single concentration scattering curve.

Iron-regulated protein FrpC (amino acids 415-591) (FrpC:415-591)
Mol. type   Protein
Organism   Neisseria meningitidis
Olig. state   Monomer
Mon. MW   18.7 kDa
 
UniProt   Q9JYV5 (415-591)
Sequence   FASTA