Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.

Prakash A, Moharana K, Wallace SS, DoubliƩ S, Nucleic Acids Res 45(5):2897-2909 (2017) Europe PMC

SASDBC7 – Human NEI like DNA glycosylase 1 (NEIL1)

Endonuclease 8-like 1
MWI(0) 51 kDa
MWexpected 45 kDa
VPorod 81 nm3
log I(s) 4.31×100 4.31×10-1 4.31×10-2 4.31×10-3
Endonuclease 8-like 1 small angle scattering data  s, nm-1
ln I(s)
Endonuclease 8-like 1 Guinier plot ln 4.31×100 Rg: 3.6 nm 0 (3.6 nm)-2 s2
Endonuclease 8-like 1 Kratky plot 1.104 0 3 sRg
Endonuclease 8-like 1 pair distance distribution function Rg: 3.8 nm 0 Dmax: 15 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Endonuclease 8-like 1 GASBOR model

Synchrotron SAXS data from solutions of Human NEI like DNA glycosylase 1 (NEIL1) in 25mM HEPES 300mM NaCl 1mM DTT 10% Glycerol, pH 7.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a MAR 165 CCD detector at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 2 mg/ml were measured at 10°C. 14 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Experimental MW reported here is calculated from Gnom based I(0) estimates and will be used for publication purpose in future. Guinier I(0) estimates are prone to presence of aggregates/radiation damage and that is why Gnom based I(0) was preferred to calculate the experimental MW.

Endonuclease 8-like 1
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   44.7 kDa
UniProt   Q96FI4
Sequence   FASTA