Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.

Prakash A, Moharana K, Wallace SS, Doublié S, Nucleic Acids Res 45(5):2897-2909 (2017) Europe PMC

SASDBD7 – Human Proliferating Cell Nuclear Antigen (PCNA)

Proliferating cell nuclear antigen
MWexperimental 86 kDa
MWexpected 89 kDa
VPorod 128 nm3
log I(s) 4.83×102 4.83×101 4.83×100 4.83×10-1
Proliferating cell nuclear antigen small angle scattering data  s, nm-1
ln I(s)
Proliferating cell nuclear antigen Guinier plot ln 4.83×102 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Proliferating cell nuclear antigen Kratky plot 1.104 0 √3 sRg
p(r)
Proliferating cell nuclear antigen pair distance distribution function Rg: 3.4 nm 0 Dmax: 9.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Proliferating cell nuclear antigen GASBOR model
Synchrotron SAXS data from solutions of Human Proliferating Cell Nuclear Antigen (PCNA) in 25mM HEPES 100mM NaCl 1mM DTT, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.4 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 6 mg/ml were measured at 10°C. 24 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Experimental MW reported here is from SAXSMoW. PCNA was used to calibrate forward intensity for MW estimation of other scatterers.

Proliferating cell nuclear antigen
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Trimer
Mon. MW   29.7 kDa
 
UniProt   P12004
Sequence   FASTA