Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20.

Otrelo-Cardoso AR, Nair RR, Correia MAS, Cordeiro RSC, Panjkovich A, Svergun DI, Santos-Silva T, Rivas MG, Sci Rep 2017 Jul 19;7(1):5798 PubMed

SASDBD9 – ABC transporter periplasmic substrate-binding protein TupA measured in the absence of metal ligand (tungstate or molybdate)

ABC transporter periplasmic substrate-binding protein
MWI(0) 29.1 kDa
MWexpected 30 kDa
VPorod 51.4 nm3
log I(s) 8.55×102 8.55×101 8.55×100 8.55×10-1
ABC transporter periplasmic substrate-binding protein small angle scattering data  s, nm-1
ln I(s)
ABC transporter periplasmic substrate-binding protein Guinier plot ln 8.55×102 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
ABC transporter periplasmic substrate-binding protein Kratky plot 1.104 0 3 sRg
p(r)
ABC transporter periplasmic substrate-binding protein pair distance distribution function Rg: 2.6 nm 0 Dmax: 9.6 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
ABC transporter periplasmic substrate-binding protein SREFLEX model
Synchrotron SAXS data from solutions of TupA measured in the absence of metal ligand (tungstate or molybdate) in 5 mM Tris-HCl, pH 7.6 were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 6 mg/ml were measured at 7°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

TupA protein measured without ligand, fitted against the apo-form model.

ABC transporter periplasmic substrate-binding protein
Mol. type   Protein
Organism   Desulfovibrio alaskensis (strain G20)
Olig. state   Monomer
Mon. MW   29.7 kDa
 
UniProt   Q316W1
Sequence   FASTA
 
PDB code   5MY5