data from solutions of
Wild-type LytA (N-His6) with choline
20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH 7.5
were collected on the
EMBL X33 camera
at the DORIS III storage ring
using a Pilatus 1M-W detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
, the range of momentum transfer 0.070 < s < 6.000 nm-1 was covered
(l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1 and 5 mg/ml were measured
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
The full-length LytA dimer adopts a rigid V-shaped scaffold with the two amidase domains in the trans configuration.
|Lytic Amidase with choline