Structural analysis reveals the flexible C-terminus of Nop15 undergoes rearrangement to recognize a pre-ribosomal RNA folding intermediate.

Zhang J, Gonzalez LE, Hall TMT, Nucleic Acids Res 45(5):2829-2837 (2017) Europe PMC

SASDBG6 – Ribosome biogenesis protein 15 (Nop15)

Ribosome biogenesis protein 15
MWexperimental 16 kDa
MWexpected 17 kDa
VPorod 38 nm3
log I(s) 1.13×102 1.13×101 1.13×100 1.13×10-1
Ribosome biogenesis protein 15 small angle scattering data  s, nm-1
ln I(s)
Ribosome biogenesis protein 15 Guinier plot ln 1.14×102 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Ribosome biogenesis protein 15 Kratky plot 1.104 0 √3 sRg
p(r)
Ribosome biogenesis protein 15 pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ribosome biogenesis protein 15 EOM/RANCH model
Ribosome biogenesis protein 15 EOM/RANCH model
Ribosome biogenesis protein 15 EOM/RANCH model
Synchrotron SAXS data from solutions of ribosome biogenesis protein 15 (Nop15) in 25 mM HEPES, 500 mM NaCl, 2 mM DTT, pH 7.5 were collected on the BL 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS, Berkeley, CA, USA) using a MAR Image Plate detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.7 and 3 mg/ml were measured at 4 °C (the data displayed in this entry: 0.7 mg/ml). One 0.5 second frame was collected per sample. The data were normalized to the intensity of the transmitted beam, radially averaged and the scattering of the solvent-blank was subtracted.

Ribosome biogenesis protein 15 (Nop15)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Monomer
Mon. MW   16.5 kDa
 
UniProt   P53927 (81-220)
Sequence   FASTA
 
PDB ID   5T9P