Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins

Pasquevich M, Dreon M, Qiu J, Mu H, Heras H, Scientific Reports 2017 Dec 7(1) DOI

SASDBJ7 – Pomacea maculata perivitellin 1

Pomacea maculata perivitellin 1
MWexperimental 294 kDa
MWexpected 278 kDa
VPorod 537 nm3
log I(s) 1.90×10-3 1.90×10-4 1.90×10-5 1.90×10-6
Pomacea maculata perivitellin 1 small angle scattering data  s, nm-1
ln I(s)
Pomacea maculata perivitellin 1 Guinier plot ln 1.90×10-3 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Pomacea maculata perivitellin 1 Kratky plot 1.104 0 3 sRg
p(r)
Pomacea maculata perivitellin 1 pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.3 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Pomacea maculata perivitellin 1 DAMMIF model
log I(s)
 s, nm-1
Pomacea maculata perivitellin 1 DAMMIF model
log I(s)
 s, nm-1
Pomacea maculata perivitellin 1 DAMMIF model
Synchrotron SAXS data from Pomacea maculata perivitellin 1 in 100 mM phosphate Buffer, pH 7.4 were collected at the SAXS2 Beamline on the Brazilian Synchrotron Light Laboratory storage ring (Campinas, Brazil) using a MAR 165 CCD detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 1.55 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1.4 and 0.7 mg/ml were measured at 25°C. Five successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.

Pomacea maculata perivitellin 1 (PmPV1)
Mol. type   Protein
Organism   Pomacea maculata (Perry, 1810)
Olig. state   Dodecamer
Mon. MW   23.2 kDa
Sequence   FASTA