Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein.

Li YC, Naveen V, Lin MG, Hsiao CD, J Biol Chem 292(38):15744-15757 (2017) PubMed

SASDBK9 – Geobacillus stearothermophilus DnaB full-length

Geobacillus stearothermophilus DnaB full-length
MWI(0) 199 kDa
MWexpected 214 kDa
VPorod 432 nm3
log I(s) 1.91×10-1 1.91×10-2 1.91×10-3 1.91×10-4
Geobacillus stearothermophilus DnaB full-length small angle scattering data  s, nm-1
ln I(s)
Geobacillus stearothermophilus DnaB full-length Guinier plot ln 1.91×10-1 Rg: 5.7 nm 0 (5.7 nm)-2 s2
(sRg)2I(s)/I(0)
Geobacillus stearothermophilus DnaB full-length Kratky plot 1.104 0 3 sRg
p(r)
Geobacillus stearothermophilus DnaB full-length pair distance distribution function Rg: 5.7 nm 0 Dmax: 20 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Geobacillus stearothermophilus DnaB full-length GASBOR model
Synchrotron SAXS data from solutions of Geobacillus stearothermophilus DnaB full-length in 20 mM Tris, 300 mM NaCl and 5 mM β-ME, pH 8 were collected on the 23A beam line at the Taiwan Photon Source storage ring (NSRRC, Hsinchu, Taiwan) using a Flat Panel sensor C10158DK-3957(X) detector at a wavelength of λ = 0.08285 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 2 and 10 mg/ml were measured at 15°C. 10 successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Geobacillus stearothermophilus DnaB full-length (GstDnaBFL)
Mol. type   Protein
Organism   Geobacillus stearothermophilus
Olig. state   Tetramer
Mon. MW   53.5 kDa
 
UniProt   A0A0K2H6I1
Sequence   FASTA