SASDBL9 – Putative DNA binding protein YeeF-CT from Bacillus subtilis (amino acids 528-669)

Putative DNA binding protein

MW^experimental	34	kDa
MW^expected	37	kDa
V^Porod	55	nm³

log I(s) 5.14×10⁴ 5.14×10³ 5.14×10² 5.14×10¹

Putative DNA binding protein small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Putative DNA binding protein Guinier plot

ln 5.15×10⁴ R_g: 2.7 nm 0 (2.7 nm)^-2 s²

(sR_g)²I(s)/I(0)

Putative DNA binding protein Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 2.7 nm 0 D_max: 9.5 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.4

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.007
0.479

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Putative DNA binding protein DAMMIN model

SAXS data from solutions of the putative DNA binding protein YeeF-CT from Bacillus subtilis (amino acids 528-669) in 20 mM HEPES 150 mM NaCl, pH 7.5 were collected on the IMTECH camera (Chandigarh, India) using a Diode 1D CMOS Mythen detector at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and where 2θ is the scattering angle). A single solute concentration of 5 mg/ml was measured at 25°C for 1800 seconds. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to produce the final scattering profile for this entry.

Sample detector distance = UNKNOWN

Putative DNA binding protein (YeeF-CT)
Mol. type		Protein
Organism		Bacillus subtilis subsp. spizizenii
Olig. state		Dimer
Mon. MW		18.5 kDa

UniProt		E0TU96 (528-669)
Sequence		FASTA