TIA-1 RRM23 binding and recognition of target oligonucleotides

Waris S, García-Mauriño S, Sivakumaran A, Beckham S, Loughlin F, Gorospe M, Díaz-Moreno I, Wilce M, Wilce J, Nucleic Acids Research 2017 Feb 10 DOI

SASDBR6 – Nucleolysin TIA-1 isoform p40 in complex with UUUUUACUCC RNA

Nucleolysin TIA-1 isoform p40
RNA (UUUUUACU)
MWexperimental 23.8 kDa
MWexpected 22 kDa
VPorod 26.5 nm3
log I(s) 5.60×10-2 5.60×10-3 5.60×10-4 5.60×10-5
Nucleolysin TIA-1 isoform p40 RNA (UUUUUACU) small angle scattering data  s, nm-1
ln I(s)
Nucleolysin TIA-1 isoform p40 RNA (UUUUUACU) Guinier plot ln 5.60×10-2 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleolysin TIA-1 isoform p40 RNA (UUUUUACU) Kratky plot 1.104 0 3 sRg
p(r)
Nucleolysin TIA-1 isoform p40 RNA (UUUUUACU) pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.5 nm

Experimental data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Nucleolysin TIA-1 isoform p40 in complex with RNA (UUUUUACUCC) in 20 mM HEPES, 100mM NaCl, 3% v/v glycerol, pH 7 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The data were collected as 30 successive 1 second frames and were normalized to the intensity of the transmitted beam and radially averaged. The radially-averaged scattering of the solvent-blank was subtracted to produce the results displayed in this entry that shows the scattering profile from a sample at 2.5 mg/ml maintained at 15°C during the SAXS measurements.

TIA-1 RRM23 + UC-rich RNA

Nucleolysin TIA-1 isoform p40 (TIA-1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.3 kDa
 
UniProt   p31483-2 (93-274)
Sequence   FASTA
 
RNA (UUUUUACU)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   0.9 kDa
Sequence   FASTA