Bovine Serum Albumin measured by SEC-SAXS

Martha Brennich.

SASDBT4 – Bovine serum albumin monomer (measured by SEC-SAXS)

Bovine serum albumin, monomer
MWexperimental 58 kDa
MWexpected 66 kDa
VPorod 118 nm3
log I(s) 1.50×102 1.50×101 1.50×100 1.50×10-1
Bovine serum albumin, monomer small angle scattering data  s, nm-1
ln I(s)
Bovine serum albumin, monomer Guinier plot ln 1.51×102 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Bovine serum albumin, monomer Kratky plot 1.104 0 3 sRg
p(r)
Bovine serum albumin, monomer pair distance distribution function Rg: 2.8 nm 0 Dmax: 8.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Bovine serum albumin, monomer PDB model

Synchrotron SAXS data from solutions of monomeric bovine serum albumin in 20 mM Tris, 142 mM NaCl, 5 % Glycerol, 1mM DTT, pH 7 were collected on the BM29 SAXS beam line at the ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.099 nm). Forty successive 1 s frames were collected through a BSA elution peak from a size-exclusion chromatography column (GE Healthcare Superdex 200 10/300 GL at 0.5 ml/min; initial load concentration = 10 mg/ml). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank (derived from the SEC running solvent) was subtracted and the individual solvent-corrected SAXS profiles were scaled and averaged.

Bovine serum albumin, monomer (BSA monomer)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   66.5 kDa
 
UniProt   P02769 (25-607)
Sequence   FASTA
 
PDB code   3V03