Asymmetric unwrapping of nucleosomal DNA propagates asymmetric opening and dissociation of the histone core.

Chen Y, Tokuda JM, Topping T, Meisburger SP, Pabit SA, Gloss LM, Pollack L, Proc Natl Acad Sci U S A 114(2):334-339 (2017) Europe PMC

SASDBT7 – Nucleosome Core Particle: 1.9 M NaCl, 50% sucrose, 50ms - 10s time resolved data

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer
Nucleasome Core Particle with Widom 601 DNA - dsDNA
MWI(0) 81 kDa
MWexpected 186 kDa
log I(s) 1.46×105 1.46×104 1.46×103 1.46×102
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA small angle scattering data  s, nm-1
ln I(s)
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Guinier plot ln 1.46×105 Rg: 6.8 nm 0 (6.8 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer Nucleasome Core Particle with Widom 601 DNA - dsDNA Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Nucleosome Core Particle in 20 mM Tris-Cl, 0.1 mM EDTA, 0.1 mM DTT, 50% sucrose, 1.9 M NaCl, pH 7.5 were collected on the 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.125 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C through a time-course spanning 50 ms to 10 s (with a 50 ms delay time). The data displayed in this entry represent the scattering after 50 ms; the remaining data from the time series can be located in the full-entry zip archive.

From the depositor: "Protein signal is blanked out in 50% sucrose, only DNA contributes to the scattering. Hence, the SAXS profile reports the Rg and molecule weight of the DNA in the nucleosome core particle complex."

Nucleasome Core Particle with Widom 601 DNA - HISTONE H2A-H2B Heterodimer
Mol. type   Protein
Organism   Xenopus laevis
Olig. state   Dimer
Mon. MW   23.8 kDa
Sequence   FASTA
 
Nucleasome Core Particle with Widom 601 DNA - HISTONE H3-H4 Heterodimer
Mol. type   Protein
Organism   Xenopus laevis
Olig. state   Dimer
Mon. MW   23.2 kDa
Sequence   FASTA
 
Nucleasome Core Particle with Widom 601 DNA - dsDNA
Mol. type   DNA
Organism   Xenopus laevis
Olig. state   Monomer
Mon. MW   92.1 kDa
Sequence   FASTA