Apple Snail Perivitellin Precursor Properties Help Explain Predators' Feeding Behavior.

Cadierno MP, Dreon MS, Heras H, Physiol Biochem Zool 2017 Jul Aug;90(4):461-470 PubMed

SASDBW4 – Albumen gland precursor of ovorubin (lipoglycocarotenoprotein agPcOvo: an oligomeric protein)

Perivitellin ovorubin-1
Perivitellin ovorubin-2
Perivitellin ovorubin-3
MWexperimental 300 kDa
MWexpected 81 kDa
VPorod 526 nm3
log I(s) 1.10×10-3 1.10×10-4 1.10×10-5 1.10×10-6
Perivitellin ovorubin-1 Perivitellin ovorubin-2 Perivitellin ovorubin-3 small angle scattering data  s, nm-1
ln I(s)
Perivitellin ovorubin-1 Perivitellin ovorubin-2 Perivitellin ovorubin-3 Guinier plot ln 1.10×10-3 Rg: 4.3 nm 0 (4.3 nm)-2 s2
(sRg)2I(s)/I(0)
Perivitellin ovorubin-1 Perivitellin ovorubin-2 Perivitellin ovorubin-3 Kratky plot 1.104 0 3 sRg
p(r)
Perivitellin ovorubin-1 Perivitellin ovorubin-2 Perivitellin ovorubin-3 pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.9 nm
log I(s)
 s, nm-1
Perivitellin ovorubin-1 Perivitellin ovorubin-2 Perivitellin ovorubin-3 DAMMIF model
log I(s)
 s, nm-1
Perivitellin ovorubin-1 Perivitellin ovorubin-2 Perivitellin ovorubin-3 DAMMIF model
X-ray synchrotron radiation scattering data from a solution of the ovorubin albumen gland precursor in 20 mM Tris-HCl, pH 8.5 were collected on the D02A-SAXS2 Beamline at the Laboratorio Nacional de Luz Sincrotron (LNLS, Campinas, Brazil) using a MAR Image Plate detector (I(s) vs s, where s = 4π sin θ/λ, 2θ is the scattering angle and λ the X-ray wavelength, 0.155 nm). Different solute concentrations in the range 1.04-4.16 mg/ml were measured. Five successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were obtained from a single concentration scattering curve.

AgPcOvo is an oligomeric lipoglycocarotenoprotein isolated from the albumen gland of P. canaliculata that consists of different perivitellin ovorubin subunits (Ovo1-3) that form a complex with a molecular weight of 300 kDa.

Perivitellin ovorubin-1 (Ovo1)
Mol. type   Protein
Organism   Pomacea canaliculata
Olig. state   Unknown
Mon. MW   22.4 kDa
 
UniProt   J7I2T6
Sequence   FASTA
 
Perivitellin ovorubin-2 (Ovo2)
Mol. type   Protein
Organism   Pomacea canaliculata
Olig. state   Unknown
Mon. MW   23.8 kDa
 
UniProt   J7HZ90
Sequence   FASTA
 
Perivitellin ovorubin-3 (Ovo3)
Mol. type   Protein
Organism   Pomacea canaliculata
Olig. state   Unknown
Mon. MW   35.0 kDa
 
UniProt   J7I5Z5
Sequence   FASTA