Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC

Liu Y, Bhowmick T, Liu Y, Gao X, Mertens H, Svergun D, Xiao J, Zhang Y, Wang J, Meijers R, Neuron (2018) DOI

SASDBZ6 – Draxin

MWI(0) 44 kDa
MWexpected 45 kDa
VPorod 87 nm3
log I(s) 3.20×10-2 3.20×10-3 3.20×10-4 3.20×10-5
Draxin small angle scattering data  s, nm-1
ln I(s)
Draxin Guinier plot ln 3.20×10-2 Rg: 4.2 nm 0 (4.2 nm)-2 s2
Draxin Kratky plot 1.104 0 3 sRg
Draxin pair distance distribution function Rg: 4.4 nm 0 Dmax: 15 nm

Experimental data validation

Fits and models

log I(s)
 s, nm-1
Draxin Rg histogram Rg, nm
Draxin EOM/GAJOE model
Draxin EOM/GAJOE model
Draxin EOM/GAJOE model
Draxin EOM/GAJOE model
Draxin EOM/GAJOE model
Draxin EOM/GAJOE model
Synchrotron SAXS data from solutions of Draxin in 20 mM HEPES 150 mM NaCl, pH 7.5 were collected on the P12 camera at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 1.24 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 1.2 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway

Draxin (Draxin)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   44.5 kDa
UniProt   Q8NBI3
Sequence   FASTA