Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase.

Reckel S, Gehin C, Tardivon D, Georgeon S, Kükenshöner T, Löhr F, Koide A, Buchner L, Panjkovich A, Reynaud A, Pinho S, Gerig B, Svergun D, Pojer F, Güntert P, Dötsch V, Koide S, Gavin AC, Hantschel O, Nat Commun 8(1):2101 (2017) PubMed

SASDC26 – DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210

BCR-ABL p210 fusion protein (DH-PH)
MWI(0) 41 kDa
MWexpected 47 kDa
VPorod 69 nm3
log I(s) 1.00×104 1.00×103 1.00×102 1.00×101
BCR-ABL p210 fusion protein (DH-PH) small angle scattering data  s, nm-1
ln I(s)
BCR-ABL p210 fusion protein (DH-PH) Guinier plot ln 1.01×104 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
BCR-ABL p210 fusion protein (DH-PH) Kratky plot 1.104 0 3 sRg
p(r)
BCR-ABL p210 fusion protein (DH-PH) pair distance distribution function Rg: 2.2 nm 0 Dmax: 11.1 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210 Rg histogram Rg, nm
BCR-ABL p210 fusion protein (DH-PH) RANCH/EOM model
BCR-ABL p210 fusion protein (DH-PH) RANCH/EOM model
BCR-ABL p210 fusion protein (DH-PH) RANCH/EOM model
BCR-ABL p210 fusion protein (DH-PH) RANCH/EOM model
Synchrotron SAXS data from solutions of DH-PH - Dbl-homology domain (DH) and Pleckstrin-homology (PH) of Bcr-Abl tyrosine kinase p210 in 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT, pH 7.5 were collected on the P12 camera at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.8 and 14.4 mg/ml were measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

BCR-ABL p210 fusion protein (DH-PH)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   47.1 kDa
 
UniProt   P11274
Sequence   FASTA