|
Synchrotron SAXS
data from solutions of
Saposin nanoparticle (SapNP) SapA:DOPC
in
PBS, pH 7.4
were collected on the
EMBL P12 camera
at the PETRA III storage ring
(Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.124 nm
, the range of momentum transfer 0.100 < s < 4.600 nm-1 was covered
(l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
|
| saposin-a
(sapA)
|
| Mol. type |
|
Protein |
| Organism |
|
Homo sapiens |
| Olig. state |
|
Monomer |
| Mon. MW |
|
9.1 kDa |
| |
| UniProt |
|
P07602 (60-140) |
| Sequence |
|
FASTA |
| |
| 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC)
|
| Mol. type |
|
Other |
| Organism |
|
Synthetic compound |
| Olig. state |
|
Other |
| Mon. MW |
|
0.8 kDa |
|
s, nm-1
